Molecular Cloning and Characterization of a Cytokinin Dehydrogenase Gene from Upland Cotton
May 20, 2011| |
Cytokinins are plant hormones that function in plant growth and development. Cytokinin dehydrogenease, known as the main negative regulator in cytokinin metabolism of plants, irreversibly degrades cytokinins into adenine/adenosine moiety. Qi-wei Zeng, researcher at Southwest University, China, and colleagues cloned a CKX homologous gene (GhCKX) from upland cotton. The transgenic tobacco plants over-expressing GhCKX exhibited a typical cytokinin-deficient phenotype, while the plants with silenced CKX showed over-producing phenotype.
Reduction in cytokinin led to a significant decrease in fiber initials on a single ovule. Results of the study indicate that GhCKX codes for a functional CKX, and cytokinins could be necessary in the initiation of cotton fiber cells.
The paper published at the Plant Molecular Biology Reporter is available at http://www.springerlink.com/content/64576658gj726184/.
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