Digital CRISPR-Cas‐Assisted Assay for Rapid SARS‐CoV‐2 DetectionNovember 25, 2020
Johns Hopkins University researchers developed the first digital CRISPR-Cas‐assisted assay called digitization‐enhanced CRISPR/Cas‐assisted one‐pot virus detection (deCOViD) which is designed for SARS‐CoV‐2, the virus causing COVID-19.
With the COVID-19 pandemic, there has been an unprecedented demand for rapid diagnostics. CRISPR-Cas-assisted nucleic acid detection assays have been found to be helpful because of their well-designed detection mechanism, fast assay time, and low reaction temperature. To further improve the technique, powerful, digital-based detection has been integrated.
According to the researchers, deCOViD is realized through tuning and discretizing a one‐step, fluorescence‐based, CRISPR-Cas12a‐assisted reverse transcription recombinase polymerase amplification assay into sub‐nanoliter reaction wells within commercially available microfluidic digital chips. With the uniformly elevated digital concentrations, deCOViD could complete qualitative detection in less than 15 minutes and quantitative detection in 30 minutes, which makes it one of the fastest and most sensitive CRISPR-Cas‐assisted SARS‐CoV‐2 detection to date.
Read more from Advanced Science.
You might also like:
- CRISPR Technique 90% Effective in Reducing SARS-CoV-2 Coronavirus
- Hospital in Israel Starts Trials for the Fastest COVID-19 Test
- Washington University Saliva Test for COVID-19 Faster and Easier
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Online Animal Biotech Discourses Cover Market and Public Readiness
- International Research Group Confirms Contribution of Bioenergy to Climate Change Mitigation
- FAO Urges G20 to Support Farmers, Protect the Vulnerable and Invest in Innovation
- OFAB Nigeria Celebrates Exemplary Science Journalism
- International Research Team Identifies Genetics Behind Deadly Oat Blight
- Study Details Efforts to Predict Corn Traits Based on Genomic and Data Analytics
- Researchers Reveal Switch Used in Plant Defense against Animal Attack
- Giant Virus Genomes Found Riding on Algae Genomes
- Biotech Experts to Tackle the Role of Biotech in Indonesia's Food Security
- EFSA Publishes Scientific Opinion on Two-Event Stack Soybean DAS-81419-2 × DAS-44406-6
- GM Maize Feed Safe For Quails
- GmMYB3a Overexpression Improves Drought Tolerance in Peanuts
- Digital CRISPR-Cas‐Assisted Assay for Rapid SARS‐CoV‐2 Detection
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: