Researchers Find Soybean Genes for Alkaline Stress ResistanceJuly 18, 2018
Sucrose non-fermenting related protein kinases (SnRKs) play important roles in plant growth, development, metabolism and resistance to environmental stresses. The soybean genome has four SnRK1 genes, of which GmSnRK1.1 and GmSnRK1.2 are predominant and participate in multiple stress response pathways. To study the role of these genes in response to ABA and alkaline stresses, a research team from the Northeast Agricultural University in China used CRISPR-Cas9 to knock out both GmSnRK1.1 and GmSnRK1.2.
Analysis of the genome-edited soybeans showed that both genes were efficiently knocked out in 48.6% of the resulting hairy roots. The team, led by Hui Qing Li, also generated control hairy roots that overexpressed GmSnRK1. The wildtypes, knockout lines, and overexpressing lines were all treated with abscissic acid for 15 days. Results showed that the growth of the wildtypes and GmSnRK1-overexpressing roots were significantly inhibited compared to the double-knockout roots. The overexpressing roots also notably displayed less root lengths and fresh weights.
However, after treatment with sodium bicarbonate for 15 days, the growth of the double-knockout roots were significantly inhibited compared to the wildtype and GmSnRK1 overexpressed control roots. These results imply that knockout of GmSnRK1.1 and GmSnRK1.2 improved hairy root sensitivity to ABA but also lessened its resistance to alkaline stress.
For more information, read the article in Europe PMC.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Experts Tackle Importance of GM Crops Commercialization in Beijing
- African Scientists Challenged to Invest in Developing Communication Skills
- International Research Team Discovers Genetic Mechanism that Allows Rice to Survive Flooding
- Farm Babe Debunks Myths about GMOs
- Japanese Researchers Assemble First Accurate Buckwheat Genome
- Cornell Alliance for Science Calls for Comments for Golden Rice in PH
- Vietnamese Farmers Share Experiences in GM Maize Planting
- Socio-economic Aspect of GM Crops Highlighted in Symposium
- Australian OGTR Issues License for GM Wheat Field Trials
- EFSA Publishes Scientific Opinion on Four-Event Stack Maize Bt11 × MIR162 × 1507 × GA21
- Sugarcane Genome Has Finally Been Sequenced
- ZmNBS25 from Maize Functions as a Disease Resistance Gene Across Species
- Upregulation of Lipid Biosynthesis Increases the Oil Content in Sorghum
Plant Breeding Innovations
- Researchers Find Soybean Genes for Alkaline Stress Resistance
- FaTM6 Controls Flower Development in Strawberry
- OsIPMS1 Gene Involved in Seed Vigor and Energy Metabolism in Rice
- Chinese Scientists Develop Improved CRISPR System
From the BICs
- "Pink Bollworm Management Strategy" Book Now Available in Telugu
- CRISPR Can Speed up Nature and Change How We Grow Food
Subscribe to CBU: