CRISPR-Cas12f1: A Mini Genome Editing ToolNovember 24, 2021
ShanghaiTech University researchers and partners reported a genome editing tool called Cas12f1. Their findings are published in Nature Chemical Biology.
The RNA-guided CRISPR-associated (Cas) nucleases are handy tools for genome editing in different species. However, the large size of the commonly used Cas9 and Cas12 nucleases limits their application, especially for therapeutic purposes. Thus, the researchers developed a miniature class 2 type V-F CRISPR-Cas genome-editing system from Acidibacillus sulfuroxidans (AsCas12f1). According to the researchers, AsCas12f1 is an RNA-guided endonuclease that recognizes 5′ T-rich protospacer adjacent motifs and generates staggered double-stranded breaks to target DNA. It has been proven to be an effective genome editing tool in both bacteria and human cells using different delivery techniques such as plasmid, ribonucleoprotein, and adeno-associated virus.
Based on the findings, AsCas12f1 offers benefits in cellular delivery. This discovery could also help other researchers engineer more compact genome editing tools. In another paper, researchers from Pakistan's National Institute for Biotechnology and Genetic Engineering reported that the CRISPR-Cas12f1 system is also useful in developing transgene-free edited plants by deploying viral-based vectors and nanoparticles.
You might also like:
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- CRISPR-Cas12a Expands Genome Engineering with Six Novel Variants
- CRISPR-Cas12b/C2c1 Tested in Targeted Mutagenesis of a Dicot
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Younger Consumers Willing to Buy Fresh Fruits Developed Through Biotechnology, Study
- FAO Releases 2021 Report on State of Food and Agriculture
- UCR Scientists Solve 50-year-old Mystery behind Plant Growth
- Researchers Develop Techniques to Breed Plants with Genes from One Parent
- Scientists Develop High-Yielding, Biofortified, Drought Tolerant Transgenic Chickpea
- ISAAA Webinar Highlights Importance of Public-Private Dynamics in Agribiotechnology Industry
- Transgenic Tobacco Becomes Pheromone Factory to Control Pests
- Study Finds Plant-based Alternative Food Consumption in UK Doubles in 10 Years
- Expert Tackles Sustainability Solutions of Gene Editing
- Study Shows Link between CRISPR and Mutated Cancer Cells
- CRISPR-Cas12f1: A Mini Genome Editing Tool
- Cuba's Homegrown COVID-19 Vaccines Show Promise
Read the latest:
- Biotech Updates (February 1, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to BU: