Experts Review Methods Used to Minimize Off-Target Effects in Genome EditingJuly 22, 2020
Researchers from King Fahd University of Petroleum and Minerals and Texas Tech University reviewed the latest approaches used to minimize off-target effects in genome editing such as biased or unbiased off-target detection, cytosine or adenine base editors, prime editing, dCas9, Cas9 paired nickase, ribonucleoprotein delivery and truncated gRNAs. The review article is published in Cells.
According to the article, CRISPR-Cas9 has been the most popular genome editing tool because of its accuracy, efficiency, cost-effectiveness, and ease of use compared to others. However, CRISPR-Cas9 may cause off-target effects at the genome level and off-target detection tools are also necessary. The most preferred off-target detecting biased and unbiased methods presented were Elevation and GUIDE–seq, respectively. Furthermore, choosing Cas variants is also critical to reduce the off-target effects that depend on the nature of the experiment.
Read more in MDPI.
You might also like:
- Researchers Develop a Tool to Predict Off-target Editing by CRISPR
- New Software Enables Sensitive and Customized Off-target Detection for CRISPR-Cas9
- Scientists Develop Hyper Accurate Cas9 for CRISPR
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Structural Analysis of COVID-19 Spike Protein Describes its Evolution
- Researchers Identify Possible Immune Targets in SARS-CoV-2 Genome
- Human Trials Begin for COVID-19 Plant-Based Technology Vaccine
News from Around the World
- GM Crops' Impact Creates a Halo Effect, Expert Explains
- ISAAA Launches Open-access Resources on Genome Editing
- Oat Genome Now on ARS Website
- Most Accurate Map of Pig Genome Gives Insights to Breeding Healthier Animals
- Researchers Develop Fine-tune Methods for Gene Silencing
Plant Breeding Innovations
- Scientists Use CRISPR-Cas9 for Single-nucleotide Editing in Rice
- TALENs Used to Edit Mitochondrial DNA
- Experts Review Methods Used to Minimize Off-Target Effects in Genome Editing
- Researchers Turn to CRISPR Genome Editing to Make Cyanide-Free Cassava
Read the latest:
- Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: