Development of Gene-Specific Rice Mutants via AvrXa23-based TALENs
June 7, 2017| |
Transcription activator-like effector nucleases (TALENs) are increasingly used as a tool for genome editing in a variety of organisms. Chinese researchers led by Fu-jun Wang of Guangxi University and Chinese Academy of Agriculture Sciences have previously cloned the TALE-coding gene avrXa23 from Xanthomonas oryzae pv. Oryzae and developed an AvrXa23-based assembly system for TALENs.
Now, the team used this AvrXa23-based TALENs system to induce mutations in the rice ethylene response factor (ERF) transcription factor, OsERF922, to test the efficiency of the system. A pair of TALENs (T-KJ9/KJ10) was assembled and was then used for transformation.
The team observed a 15% mutagenesis frequency in positively transformed rice calli and obtained two mutant plants with nucleotide deletion or insertion at the target region. This study shows that the AvrXa23-based TALENs system can be used for site-specific genome editing in rice.
For more on this study, read the article in Journal of Integrative Agriculture.
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