Researchers Use CRISPR/Cas9 for Precise Excision of Transgenes from Rice GenomeFebruary 1, 2017
The CRISPR/Cas9 system has emerged as a versatile gene editing tool that functions through the double-stranded break repair leading to targeted mutagenesis. This RNA-guided nuclease system has mostly been applied for inducing point mutations or short insertion-deletions at one or multiple loci.
Researchers from University of Arkansas, led by Vibha Srivastava, used CRISPR/Cas9 system for excising marker genes from plant genomes to develop marker-free transgenic plants. A transgenic rice line expressing β-glucuronidase (GUS) gene was transformed by Agrobacterium or gene gun with a construct expressing Cas9 and two guide RNAs to target each end of the GUS gene.
Analysis of the transformed lines detected excision at low frequency in the callus lines. However, a significantly higher excision frequency was detected in plant lines, indicating the efficiency of Cas9:gRNA in regenerated plants.
Marker-removal technologies aim to precisely excise a specific piece of DNA without introducing mutations, and the Cas9:gRNA system could be an effective tool for producing marker-free plants.
For more on the study, read the article in Plant Cell, Tissue and Organ Culture.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Uganda MP Wants Constituents to be First Beneficiaries of GM Crops
- AATF Establishes First Early Generation Seed Production Entity in Sub-Saharan Africa
- Research Discovers How Plants "Turn Off" Immune Response after Confronting Pathogens
- University of Florida Research Team Discovers Genetic Key to Restoring Tomato Flavor
- International Collaboration to Discover Sources of Genetic Resistance to Corn Diseases
- Study Shows Effects of Soil Salinity on Cry1Ac Expression in Bt Cotton
- Seed-specific Overexpression of BASS2 Enhances Oil Production in Arabidopsis Seeds
Plant Breeding Innovations
- Programming Antiviral Defenses in Plants through Genome Editing
- Researchers Use CRISPR/Cas9 for Precise Excision of Transgenes from Rice Genome
Beyond Crop Biotech
- Genomes of Two Human Malaria Species Revealed
- Scientists Develop New System that Use Mobile Phone to Treat Disease
- 4th Conference of Cereal Biotechnology and Breeding
- ISAAA in 2016
- The National Academies Releases Book on GE Crop Study
- Video: Hawaii GMO Papaya Real Solutions Real Lives
Read the latest:
- Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: