Seed-specific Overexpression of BASS2 Enhances Oil Production in Arabidopsis SeedsFebruary 1, 2017
Seed oil is important for human and animal nutrition as well as for various industrial applications. Genetic engineering strategies have been tried to increase oil content in seeds. However, few of these strategies involve manipulating the transporters. Pyruvate is a major source of carbon for fatty acid biosynthesis in plastids, and the embryo's demand for it is reported to increase during oil accumulation. The team of Eun-Jung Lee of Pohang University of Science and Technology in South Korea aimed to boost oil biosynthesis by increasing pyruvate flow into plastids.
The team expressed the plastid-localized pyruvate transporter BILE ACID:SODIUM SYMPORTER FAMILY PROTEIN 2 (BASS2) in Arabidopsis thaliana. The resulting transgenic Arabidopsis plants overexpressing BASS2, produced seeds that were larger and heavier. The transgenic seeds also contained 10-37% more oil than wild types despite having comparable protein and carbohydrate contents. This reflected to an increase of 24-43% in oil yield per plant.
These data demonstrate that seed-specific overexpression of the pyruvate transporter BASS2 can enhance oil production in Arabidopsis seeds. Furthermore, it also proves that manipulating specific transporters is a feasible approach for increasing the seed oil content.
For more on this study, read the article in Frontiers in Plant Science.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Uganda MP Wants Constituents to be First Beneficiaries of GM Crops
- AATF Establishes First Early Generation Seed Production Entity in Sub-Saharan Africa
- Research Discovers How Plants "Turn Off" Immune Response after Confronting Pathogens
- University of Florida Research Team Discovers Genetic Key to Restoring Tomato Flavor
- International Collaboration to Discover Sources of Genetic Resistance to Corn Diseases
- Study Shows Effects of Soil Salinity on Cry1Ac Expression in Bt Cotton
- Seed-specific Overexpression of BASS2 Enhances Oil Production in Arabidopsis Seeds
Plant Breeding Innovations
- Programming Antiviral Defenses in Plants through Genome Editing
- Researchers Use CRISPR/Cas9 for Precise Excision of Transgenes from Rice Genome
Beyond Crop Biotech
- Genomes of Two Human Malaria Species Revealed
- Scientists Develop New System that Use Mobile Phone to Treat Disease
- 4th Conference of Cereal Biotechnology and Breeding
- ISAAA in 2016
- The National Academies Releases Book on GE Crop Study
- Video: Hawaii GMO Papaya Real Solutions Real Lives
Subscribe to CBU: