CRISPR-Cas13a Shows Efficient Phage Genome EditingNovember 9, 2022
A team of researchers from California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, reported that broad-spectrum CRISPR-Cas13a enables efficient phage genome editing. Their study is published in Nature Microbiology.
CRISPR-Cas13 defend against incoming RNA and DNA phages by binding to complementary target phage transcripts followed by general, non-specific RNA degradation. The researchers analyzed the defensive capabilities of LbuCas13a from Gram-negative rod bacteria Leptotrichia buccalis and found that it had efficient antiviral activity, which is not affected by target phage gene essentiality, gene expression timing or target sequence location. Moreover, its antiviral activity was also proven to be broadly effective against a wide range of phages by challenging LbuCas13a against nine Escherichia coli phages from diverse phylogenetic groups.
Because of the observed versatility and potency of LbuCas13a targeting, LbuCas13a was tested for broad-spectrum phage editing. Using a two-step phage-editing and enrichment method, seven marker-free genome edits were achieved in three diverse phages with 100% efficiency, including small and large edits.
Read the research article in Nature Microbiology.
You might also like:
- Two Studies Reveal Retron's Function and its Genome-editing Potential
- Researchers Map Largest, Most Complex CRISPR System
- CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
Research and Tools
- Study Pinpoints Enzyme Linked to Rice Plant Height
- Genome-Edited Chickens and Ducks Produced Using Non-PGC Method
- New Technique TARDIS Makes Gene Editing at Scale Possible in Animals
- CRISPR-Cas13a Shows Efficient Phage Genome Editing
- Rice University Research Group Develops Technique to Find Large Deletions and Anomalies in ‘On-target' CRISPR-Cas9 Editing
Public Acceptance and Engagement
- Public Understands CRISPR Better with Infographics and Videos
Read the latest:
- Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to CBU: