Scientists Assess Use of Meganucleases in Tomato and Oilseed Rape
November 10, 2021
Researchers from France evaluated the use of two meganucleases, I-SceI and a customized meganuclease, in tomato and oilseed rape. Their findings are published in Transgenic Research.
Meganucleases are molecular scissors that can cause changes in the DNA. Because of this function, they are used in editing the genomes of plants. To assess their efficacy in tomato and oilseed rape, the researchers employed different strategies.
First, they used a reporter construct GFFP with the target sequences which showed that both meganucleases can generate double-strand break and HDR mediated recombination in a reporter gene. They also observed that I-SceI have a higher DSB efficiency than the customized meganuclease. DSB efficiency reached up to 62.5% in tomato and 44.8% in oilseed rape.
For the second strategy, the researchers introduced the same exogenous landing pad for tomato and oilseed rape. I-SceI turned out to be less efficient, while the customized meganuclease generated the excision of an exogenous transgene present in tomato.
Read more results in Transgenic Research.
You might also like:
- Genome Editing Resource
- What are TALENs?
- Researchers Review Targeted Genome Editing Techniques in Horticultural Crops
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
COVID-19 Alerts
- UC San Diego Researchers Develop CRISPR-based Rapid Diagnostic Tool for SARS-CoV-2
-
News from Around the World
- Svalbard Global Seed Vault Receives First Deposits from Serbia and Latvia
- UN Climate Conference Calls for Urgent Action in Agriculture
- Study Highlights Economic Loss Caused by Barriers of GM Crop Commercialization
- Genomics to Help Breeders Develop Rice with Improved Taste, Cooking Time, Texture, and Appearance
- Scientists Confirm GM Crop Cultivation and Glyphosate Use Reduce Carbon Footprint Caused by Farming
- Food Futures: Commercialization of Gene Edited Crops in Asia and Australia
- 4th Asian Short Course on Agri-biotech, Biosafety Regulation, and Communication (ASCA2021)
- Research Finds Winter Important for Cereal Yield
- Gene Discovery Yields High Promise for Wheat Fertility in Changing Climate
-
Research Highlights
- Bax Gene Confers Bacterial Blight Resistance in Rice
-
Plant Breeding Innovations
- Scientists Assess Use of Meganucleases in Tomato and Oilseed Rape
-
Read the latest: - Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
-
Subscribe to CBU: - Share
- Tweet