Researchers Present Improved Direct PCR-based Technology for GE Plants ScreeningApril 21, 2021
Researchers from the Centre of Biotechnology of Borj Cedria in Tunisia established a standard direct PCR workflow for timely gene detection assay and screening of genetically engineered (GE) plants. The results are published in Transgenic Research.
GE plants are widely developed for various practical and fundamental purposes. Thus, several methods have been developed for the detection and quantification of GE crops, but are still labor-intensive and expensive. Thus, Anis Ben-Amar and Ahmed Mliki developed a direct PCR technology using a regular Taq polymerase without prior DNA purification over a wide range of plant species. With the new method, only a small amount of the fresh tissue is necessary to amplify the target gene sequences successfully, leading to high-quality sequencing profiles. GE plant screening also confirmed the integration of the reporter gene and selectable marker into the plant genome.
Based on the findings, the improved direct PCR approach provides a powerful tool for large-scale PCR-based gene detection making DNA purification irrelevant.
You might also like:
- Pocket K No. 22: Plant Disease Diagnostics
- New Multiplex RTi-PCR Method for GMO Detection
- Simple Method for Rapid Screening of CRISPR-Cas9-Induced Mutants
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
- Experts Release Guidance on How to Communicate about COVID-19 Vaccine Efficacy
News from Around the World
- Researchers Identify Factors Affecting Critical Thinking toward GM Foods
- Brazil Approves Oxitec's Friendly™ Technology Against Fall Armyworm
- Multi-Institutional Effort Produces Comprehensive Guide for Communicating Plant Science
- Indian Farmers Lose Opportunity to Benefit from Bt Brinjal as GEAC Halts Field Trials
- JIC Study Reveals Genes that Control Growth Habit in Plants
- EFSA Report Says 98% of Pesticide Residues in Food in the EU within Legal Limits
- Studies Show How Microbiome Affects Breeding of Apples and Oil Pumpkins
- Researchers Present Improved Direct PCR-based Technology for GE Plants Screening
- Success of Bt Brinjal in Bangladesh Opens Possibility for Two New Eggplant Varieties
Plant Breeding Innovations
- Scientists Develop Gene-Edited Mosquito to Help Eliminate Malaria
- Global Gene Editing Tools Market to Continue Excellent Growth
Subscribe to CBU: