Simple Method for Rapid Screening of CRISPR-Cas9-Induced Mutants
April 24, 2019The clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas9) system is considered as a technological revolution in targeted mutagenesis. However, a large amount of time and cost is needed to screen for the CRISPR-Cas9 induced mutants from a usual large number of initial samples. Thus, Chun Wang and Kejian Wang from the Chinese Academy of Agricultural Sciences presented a cost-effective and sensitive screening technique for identifying mutants based on conventional polymerase chain reaction (PCR). They called this new technique as annealing at critical temperature PCR (ACT-PCR).
ACT-PCR needs only one PCR step and then execution of agarose gel electrophoresis. Because of its simplicity, ACT-PCR is suitable for rapid, large-scale screening of CRISPR-Cas9-induced mutants.
Read more description about ACT-PCR in Plant Genome Editing with CRISPR Systems.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Scientists Race to Save Bananas from Panama Disease
- Researchers Use Order Theory to Show the Connection Between CO2 Emission and GM Crops
- Scientists Discover Ways to Regenerate Plant Tissues
- Japanese Health Ministry Finalizes Policy on Genome-Edited Food Products
- Australian OGTR Grants Permit to GM Wheat Field Trial
- Knowing How Cells Grow and Divide Leads to More Robust and Productive Plants
-
Research Highlights
- LncRNA Controls Susceptibility to Cry1Ac in Pink Bollworm
- Salt Tolerant Rice Variety Developed in India
-
Plant Breeding Innovations
- Simple Method for Rapid Screening of CRISPR-Cas9-Induced Mutants
- Experts Release Protocol for Creating Large Chromosomal Deletions in Rice Using CRISPR-Cas9
-
Beyond Crop Biotech
- NTU Researchers Genetically Modify Key Protein in Plants to Increase Seed Oil Yield
- Scientists Explore Consumer Response to Health Information Campaigns and Genetically Modified Food Labels
-
Announcements
- CRISPRcon 2019
- CBU Subscriber Survey
-
Subscribe to CBU: