Transgene-free PDS Mutants in Potato Generated
September 23, 2020
Researchers from NARIC Agricultural Biotechnology Institute in Hungary successfully developed transgene-free potato mutants using CRISPR-Cas9 and Agrobacterium-mediated transformation. The results are published in BMC Biotechnology.
Agrobacterium-mediated gene transfer is commonly used in plants. To remove the transgene in the succeeding generations, selfing is applied by breeders. However, this method cannot be applied in several commercial tomato varieties due to self-incompatibility. Thus, the researchers tested the efficiency of a temporary expression of Cas9 system targeting the PHYTOENE DESATURASE (PDS) gene which is involved in carotenoid biosynthesis. Upon vegetative propagation from stem segments in vitro, the phenotype of the plants obtained even by positive selection of the new vector did not change, suggesting that the expression of Cas9 and gPDS is silenced or that the DNA repair system is highly active during the vegetative growth phase in potato.
Read more results in BMC Biotechnology.
You might also like:
- GM Potato Shows Improved Colorado Potato Beetle Resistance
- GM Approval Database: Potato Events
- Most Complete Potato Genome Sequence Published
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
COVID-19 Alerts
- Research Highlights COVID-19 Impacts on Food Security in Kenya and Uganda
-
News from Around the World
- FAO Facilitates Agri-food Systems Transformation of Cities Worldwide
- Ugandan Farmers Want Bt Maize to Address Fall Armyworm Challenge
- 10 New Genes Identified for Resistance to Stripe Rust Disease
- Research Team Turns to Wild Crop Relatives in Climate Change Battle
- Scientists Identify New Resistance Gene to Potato Disease that Caused Irish Famine
- Bt Cotton Increased Farmers' Produce by 10-Fold in China
-
Research Highlights
- E. coli Bacteria Offer a Path to Improving Photosynthesis
- Scientists Analyze Whole Genome Sequence of Water Buffalo, Cattle for Domestication Signatures
-
Plant Breeding Innovations
- CRISPR-Cas9 Reveals Genes Involved in Rice Heading Date
- Transgene-free PDS Mutants in Potato Generated
- TALENs Used for Genome Editing of an Algal Biodiesel Candidate
-
Subscribe to CBU: