Simultaneous and Precise Generation of Zebra3 and Wsl5 Mutations in Rice using CRISPR-Cas9October 2, 2019
Scientists at The Pennsylvania State University used Z3adenin base editors to induce rapid generation of precise point mutations in two distinct genes, OsWsl5, and OsZebra3 (Z3), in rice protoplasts and regenerated plants. The results are published in bioRXiv.
CRISPR-Cas9 can be used efficiently induce point mutations in the genome without introducing double-strand break or supplying a DNA donor template for homology-dependent repair. In the study, CRISPR-Cas9 precisely engineered point mutations which were stably inherited to subsequent generations. The single nucleotide changes led to single amino acid modifications and associated wsl5 and z3 phenotypes as manifested by white stripe leaf and light green/dark green leaf pattern, respectively. Using selfing and segregation, the researchers generated transgene-free, base edited wsl5 and z3 mutants in a short span of time. They observed a novel mutation (V540A) in locus which leads to a similar phenotype of Z3 mutation (S542P).
Based on the findings, the vectors of the adenine base editors and the methods used in the study could be used to simultaneously generate point mutations in several genes in a single transformation.
Read more results in bioRXiv.
You might also like:
- Pocket K No. 37: Biotech Rice
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- OsNramp5 Mutation Affects Cd Accumulation and Agronomic Traits in Rice
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- GM Crops Benefit Farmers' Mental Health, Consumers' Overall Health
- Balanced Reporting Promotes Agri-biotech Acceptance in Kenya
- Chilean Scientist Develops Transgenic Corn that Survives 52 Days Without Water
- Brazil Seeks Active Cooperation with Gulf States on GM Food R&D
- Researchers Discover How Protein Connecting Calcium and Hormone Regulates Plant Growth
- Three Wheat Genes Linked to Yield Gains
- SEARCA-BIC Spearheads Regional Public Briefing and Social Media Training on Agri-biotech
- Scientists Isolate and Characterize C-Repeat Binding Factor-Coding Gene from Maize
Beyond Crop Biotech
- Almond and Peach Genomes Explain Differences of Fruits and Seeds of these Closely Related Species
- Simultaneous and Precise Generation of Zebra3 and Wsl5 Mutations in Rice using CRISPR-Cas9
- Disruption of a Small Non-coding RNA Leads to Better Rice Yield
Read the latest:
- Biotech Updates (March 29, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: