Use of CRISPR-Cas9 Revealed OsCAF1 Influences Chloroplast Development in RiceSeptember 11, 2019
The chloroplast splicing and ribosome maturation (CRM) domain proteins have functions in the splicing of chloroplast gene introns. Several CRM domain proteins have been discovered to be involved in chloroplast development in many plants, but their functions in rice are still yet to be known. To elucidate more on this, scientist Qiang Zhang from Chinese Academy of Sciences and colleagues used CRISPR-Cas9 technology. The results were reported in the International Journal of Molecular Sciences.
Albino mutants oscaf1 were generated through the editing of OSCAF1 with two CRM domains using CRISPR-Cas9. The mutants eventually died at seedling stage. It was observed that the mutants had fewer chloroplast numbers and damaged chloroplast structures.OsCAF1 was found in the chloroplast, and were in high amounts in green tissues. Furthermore, OsCAF1 promoted the splicing of group IIA and group IIB introns, which could be influenced by its interaction with OsCRS2.
Read the full paper in the International Journal of Molecular Sciences.
You might also like:
- Pocket K No. 37: Biotech Rice
- GM Approval Database - Rice Events
- Golden Rice Has Same Nutrients as Traditional Rice Except for the Increased Provitamin A Content
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- GM Crop Planting Increased ~113-fold Since 1996
- Burkinabe's National Biosafety Laboratory Opened
- Students Concerned about Future of Biotech Innovations in Uganda
- Discovery of Genes Could Help Reduce Fertilizer Pollution in Waterways
- Plant Enzyme Could Guide Development of Medicines and Other Products
- Half of Australians Give ‘Conditional Support' to GM foods, Study Reveals
- Utrecht Biologists Discover A Way to Make Plants Flood Tolerant
- First Pea Genome to Help Improve Future Crops
- Scientists Investigate Grapevine's Population Genetics Using Structural Variants
Plant Breeding Innovations
- Use of CRISPR-Cas9 Revealed OsCAF1 Influences Chloroplast Development in Rice
Subscribe to CBU: