Nicotiana benthamiana as Suitable System for Expression of Cotton Boll Weevil α-amylase Inhibitor
March 20, 2019| |
Cotton boll weevil is one of the most destructive pests of cotton in the Americas. Institut de L'Environnement et de Recherches Agricoles (INERA) scientist, Severine Lacombe, and colleagues developed an α-amylase inhibitor (α-AIC3) variant using molecular evolution strategy to impede the action of cotton boll weevil (Anthonomus grandis). The findings are published in BMC Biotechnology.
The researchers produced α-AIC3 using a temporary expression system in Nicotiana benthamiana, a relative of tobacco. Structural and functional characterization of α-AIC3 showed that it has the ability to inhibit up to 100% of A. grandis α-amylase, while it had no effect on the α-amylase of two other non-pathogenic insects.
The results imply that N. bethamiana is a suitable system for high-level production of biologically active α-AIC3. The data also suggest that α-AIC3 could be a potential candidate for the production of GM crops with resistance to cotton boll weevil.
Read the research article in BMC Biotechnology.
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