CRISPR-Cas9 Used to Reduce Symptoms of Cassava Brown Streak DiseaseAugust 29, 2018
Scientist Michael Gomez from the University of California, Berkeley and colleagues aim to contribute in alleviating this problem by targeting novel cap-binding proteins (nCBP-1 and nCBP-2) for CRISPR-Cas9-mediated editing. These proteins are among the elF4E isoforms involved in the onset of CBSD.
They observed delayed and attenuated CBSD aerial symptoms and reduced severity and incidence of root necrosis, which is one of CBSV infection symptoms, in the CRISPR mutants. CRISPR-Cas9 proved to be an effective tool in promoting disease tolerance in cassava. The researchers aim to study the effect of other remaining elF4E isoforms on CBSD to further design a cassava disease tolerance strategy.
For more information, read the article in Plant Biotechnology Journal.
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