Research Team Combines Microspore Technology with CRISPR-Cas9
May 9, 2018| |
The application of gene editing techniques such as TALENs and CRISPR-Cas9 is hindered by the long life cycle of crop species. Moreover, desired genotypes generally require multiple generations to achieve. Meanwhile, single-celled microspores are haploid cells that can be developed into double haploid plants and have been widely used as a breeding tool to generate homozygous plants within a generation.
A researcher from the National Research Council Canada, Pankaj Bhowmik, and his colleagues combined the CRISPR-Cas9 system with microspore technology to develop a haploid mutagenesis system to induce genetic modifications in the wheat genome.
The team first investigated the optimal conditions for microspore transfection of the CRISPR-Cas9 into microspores. Using multiple Cas9 and sgRNA constructs, the team successfully introduced targeted mutations in the reporter gene, DsRed, as well as two endogenous wheat genes, TaLox2 and TaUbiL1. However, the regeneration efficiency from microspores is still low and requires further study.
This study demonstrates the feasibility of combining microspore technology and CRISPR-Cas9 for trait discovery and crop improvement.
For more information, read the article in Scientific Reports.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Advances in Crop Science Critical in Combating Effects of Climate Change
- New Sunflower Seeds Improve Yields in Tanzania
- Scientists Publish New Model for Communication in Plant Cells
- USDA Proposes New National Bioengineered Food Disclosure Standard
- Rice with Triple-stack Traits Shows Better Yields Amidst Abiotic Stresses
- Radiocarbon-based Study Suggests Wheat Introduced to China in 2600 BCE
- Australian OGTR Invites Comments on GM Wheat Field Trial
- Biotech Experts Push Forward Bill on Modern Biotech in PH
- New Analysis Reveals Organic Agriculture Less Productive than Conventional Agriculture
-
Research Highlights
- Scientists Reveal that a MAPK Signaling Pathway Controls Grain Size
- SlbZIP1 Regulates Biotic and Abiotic Stress Tolerance in Tomato
- Overexpression of Arabidopsis Gene Enhances Vitamin B6 Content in Potato
-
Beyond Crop Biotech
- Rose Genome Provides New Insights into the Domestication of Modern Roses
-
Announcements
- Asian Short Course on Agri-biotechnology, Biosafety Regulation, and Communication
-
Plant
- CRISPR- Mediated Mutagenesis on Duplicated Loci in Soybean
- CRISPR-Cas9 System Used to Develop Pink Tomatoes
- Rapeseed with Increased Oleic Acid Generated through CRISPR
- Research Team Combines Microspore Technology with CRISPR-Cas9
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet