Rapeseed with Increased Oleic Acid Generated through CRISPRMay 9, 2018
The CRISPR-Cas9 system has been widely used for modifying endogenous genes. Thus, it is expected to be applied for the breeding of other economically important plant species. A research team led by Ayako Okuzaki from Tamagawa University in Japan recently modified a fatty acid desaturase 2 gene (FAD2), which encodes an enzyme that catalyzes the desaturation of oleic acid, in rapeseed (Brassica napus) cultivar, Westar.
Two guide RNAs were designed to target the BnaA.FAD2.a gene and two mature plants with mutant alleles were generated. Plants with the fad2_Aa allele without any transgenes were selected from the backcross progenies and plants homozygous for fad2_Aa were then produced by self-crossing the BC1 progenies. The fatty acid composition analysis of their seeds revealed a significant increase in the content of oleic acid compared to wild types.
These results showed that the application of the CRISPR-Cas9 system is useful in producing desirable mutant plants with agronomically suitable traits by modifying their metabolic pathway.
For more information, read the article in Plant Physiology and Biochemistry.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Advances in Crop Science Critical in Combating Effects of Climate Change
- New Sunflower Seeds Improve Yields in Tanzania
- Scientists Publish New Model for Communication in Plant Cells
- USDA Proposes New National Bioengineered Food Disclosure Standard
- Rice with Triple-stack Traits Shows Better Yields Amidst Abiotic Stresses
- Radiocarbon-based Study Suggests Wheat Introduced to China in 2600 BCE
- Australian OGTR Invites Comments on GM Wheat Field Trial
- Biotech Experts Push Forward Bill on Modern Biotech in PH
- New Analysis Reveals Organic Agriculture Less Productive than Conventional Agriculture
- Scientists Reveal that a MAPK Signaling Pathway Controls Grain Size
- SlbZIP1 Regulates Biotic and Abiotic Stress Tolerance in Tomato
- Overexpression of Arabidopsis Gene Enhances Vitamin B6 Content in Potato
Plant Breeding Innovations
- CRISPR- Mediated Mutagenesis on Duplicated Loci in Soybean
- CRISPR-Cas9 System Used to Develop Pink Tomatoes
- Rapeseed with Increased Oleic Acid Generated through CRISPR
- Research Team Combines Microspore Technology with CRISPR-Cas9
Beyond Crop Biotech
- Rose Genome Provides New Insights into the Domestication of Modern Roses
- Asian Short Course on Agri-biotechnology, Biosafety Regulation, and Communication
Read the latest:
- Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to CBU: