CRISPR-Cas9 System Used to Develop Pink TomatoesMay 9, 2018
Fruit color is regarded as one of the most important commercial traits in tomato (Solanum lycopersicum). Different regions have different color preferences, with pink tomatoes being popular in Asia, particularly in China and Japan. The team of Lei Deng from the Chinese Academy of Sciences aims to develop pink tomatoes by targeting the SlMYB12 gene, a key gene in flavonoid accumulation in tomato.
The team used CRISPR-Cas9 to disrupt the SlMYB12 gene in red-fruit tomato lines. Eleven individual T0 regeneration plants were developed, 10 of which had at least one mutated SlMYB12 allele, indicating high editing efficiency (90.9%). All homozygous and bi-allelic mutants displayed a pink fruit phenotype.
No off-target mutations were detected at any of the three potential off-target sites suggesting that the sgRNAs used were specific for their recognition sites.
For more information, read the article in Journal of Genetics and Genomics.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Advances in Crop Science Critical in Combating Effects of Climate Change
- New Sunflower Seeds Improve Yields in Tanzania
- Scientists Publish New Model for Communication in Plant Cells
- USDA Proposes New National Bioengineered Food Disclosure Standard
- Rice with Triple-stack Traits Shows Better Yields Amidst Abiotic Stresses
- Radiocarbon-based Study Suggests Wheat Introduced to China in 2600 BCE
- Australian OGTR Invites Comments on GM Wheat Field Trial
- Biotech Experts Push Forward Bill on Modern Biotech in PH
- New Analysis Reveals Organic Agriculture Less Productive than Conventional Agriculture
- Scientists Reveal that a MAPK Signaling Pathway Controls Grain Size
- SlbZIP1 Regulates Biotic and Abiotic Stress Tolerance in Tomato
- Overexpression of Arabidopsis Gene Enhances Vitamin B6 Content in Potato
Plant Breeding Innovations
- CRISPR- Mediated Mutagenesis on Duplicated Loci in Soybean
- CRISPR-Cas9 System Used to Develop Pink Tomatoes
- Rapeseed with Increased Oleic Acid Generated through CRISPR
- Research Team Combines Microspore Technology with CRISPR-Cas9
Beyond Crop Biotech
- Rose Genome Provides New Insights into the Domestication of Modern Roses
- Asian Short Course on Agri-biotechnology, Biosafety Regulation, and Communication
Read the latest:
- Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to CBU: