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Crop Biotech Update

Arabidopsis' AtGOLS1 Gene Negatively Regulates Seed Germination

December 13, 2017

Seed germination begins the growth phases of plants and its rate is affected by plant hormones such as abscisic acid (ABA), gibberellin (GA) and brassinosteroids (BRs), as well as by environmental factors. The team of Ji-Hye Jang of Sookmyung Women's University in South Korea searched for additional reagents that may affect seed germination using the det2-1 and ga1-3 mutants, which showed reduced seed germination due to their defective BR- or GA- synthesis, respectively.

The team found that dithiothreitol (DTT) specifically enhanced seed germination of det2-1 mutant. The team then identified and focused on the Arabidopsis gene, AtGOLS1, as a differentially expressed gene between the mutants and those treated with DTT.

AtGOLS1 encodes a galactinol synthase, which is critical for the oligosaccharide synthesis during seed maturation. The expression of AtGOLS1 was found to decrease when conditions were favorable for seed germination. Furthermore, seed germination rate was faster in AtGOLS1-knockout mutants compared with wild type plants.

These results suggest that AtGOLS1 acts as a negative regulator in seed germination in Arabidopsis.

For more on this study, read the article in Plant Science.