DND1-Silencing in Potato and Tomato Confers Resistance to Botrytis cinereaDecember 13, 2017
Botrytis cinerea infects many crops including potato and tomato. Major genes for complete resistance to B. cinerea are not known, but a few quantitative trait loci (QTL) have been described in tomato. Previous studies have also proven that the loss of function of particular susceptibility (S) genes may provide resistance to B. cinerea in Arabidopsis.
Kaile Sun Wageningen University & Research used RNAi to silence the orthologs of Arabidopsis S genes DND1, DMR6, DMR1 and PMR4 in potato and the DND1 ortholog in tomato. The DND1-silenced potato and tomato plants showed significantly reduced B. cinerea leaf lesions as compared to control plants. Reduced lesions were also observed on the leaves of DMR6-silenced potato plants but only at 3 days after inoculation.
On the other hand, the DMR1 and PMR4-silenced potato transformants were still susceptible to B. cinerea as the control plants. Microscopic analysis in DND1-silenced potato and tomato leaves revealed that a significantly lower number of B. cinerea conidia remained attached to the leaf surface of the DND1-silenced potato and tomato plants. Moreover, the growth of the hyphae was also hindered.
Silencing of DND1 led to reduced susceptibility to Botrytis, which was found to be related to the impediment of conidial germination and attachment as well as hyphae growth. These provide new insights regarding the use of S genes in resistance breeding.
For more on this study, read the article in BMC Plant Biology.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO Calls for Urgent Actions to Reverse the Rise in Hunger
- Global Impact of Not Planting Herbicide Tolerant Crops Due to Glyphosate Ban
- Study Identifies Genetic Basis of Western Corn Rootworm Resistance
- New Technique to Help Plant Breeders Develop Drought Resistant Varieties Faster
- Australian OGTR Invites Comments for Field Trial of GM Perennial Ryegrass
- Max Planck Researchers Engineer Key Enzyme in Photosynthesis
- DND1-Silencing in Potato and Tomato Confers Resistance to Botrytis cinerea
- ThPP1 Gene Enhances Alkali Stress Tolerance in Transgenic Rice
- Arabidopsis' AtGOLS1 Gene Negatively Regulates Seed Germination
Plant Breeding Innovations
- Indian Researchers Test CRISPR-Cas9 Genome Editing in Banana
- Lignin Reduction Observed in CRISPR-Edited Switchgrass
Beyond Crop Biotech
- Salk Scientists Use Modified CRISPR System to Treat Diseases
- Sugarcane in Africa
- Biotech Crop and Traits Annual Updates
- ISAAA Blog: Clive James and ISAAA, Top Sources of Info on Biotech in the Philippines
Read the latest:
- Crop Biotech Update (August 17, 2022)
- Genome Editing Supplement (August 10, 2022)
- Gene Drive Supplement (July 27, 2022)
Subscribe to CBU: