CRISPR-Cas9 and TALENs Used to Generate Mutants for Studying Small RNA in LegumesDecember 6, 2017
Processing of double-stranded RNA precursors is essential in regulating gene expression in plant development and stress response. Small RNA processing requires the activity of a diverse group of components. However, in most plant species, there are insufficient mutant resources to study each component. Hence, the team of Shaun J. Curtin from the University of Minnesota generated legume mutants that represent mutations in each loci involved in small RNA processing in soybean (Glycine max) and alfalfa (Medicago truncatula) using CRISPR-Cas9 and TALENs platforms.
A CRISPR-Cas9 reagent was used to create a bi-allelic double mutant for the two soybean paralogs for the Double-stranded RNA-binding2 gene (Gmdrb2ab mutant). Meanwhile, a CRISPR-Cas9-generated alfalfa mutant without the Hua enhancer1 (MtHen1) gene was also developed. All mutations were found to be transmissible.
On the other hand, TALENs was used to generate a mutation within the soybean Dicer-like2 gene. CRISPR-Cas9 mutagenesis of the soybean Dicer-like3 gene and the GmHen1a gene was observed, but were not transmitted to the next generation.
Finally, a set of mutant combinations was generated by combining the previously reported mutants with the Gmdrb2ab double mutant.
This study demonstrates the use of genome engineering platforms to generate a collection of useful mutant plant lines for future study of small RNA processing in legume crops.
For more on this study, read the article in Plant Biotechnology Journal.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO: Urgent Action Needed to Combat Fast-spreading Pests and Diseases that Harm Food Security
- Plant and Animal Innovation Experts Discuss Practical Strategies to Advance Plant Breeding
- African Agri Researchers Trained on Science Communication
- Research Team from US, China, and France Releases Genome Sequence of Bottle Gourd
- Plant Biologist Wins the 2018 Breakthrough Prize in Life Sciences
- Genes from Drought Resistant Plants Could Help Create Water Efficient Crops
- Researchers Discover Gene for Flowering also Directs Root Growth in Wheat and Barley
- Study Shows that Golden Rice Could Benefit Bangladesh
- USDA FAS Releases Annual Agbiotech Report for Japan
- Glyphosate Gets 5-Year Renewal in the European Union
- European Science Academies Call for Urgent Action on Food and Nutrition Security
- Arabidopsis Elongator Genes Enhance Disease Resistance in Strawberry
- miR172c and NNC1 Regulate Root Plastic Development in Response to Salt Stress in Soybean
- Biotech Country Facts and Trends
- CRISPR-Cas9 and TALENs Used to Generate Mutants for Studying Small RNA in Legumes
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: