Evaluation of HvPAPhy_a Gene in Barley Using CRISPR-Cas9 and TALENsAugust 9, 2017
Aarhus University's Inger B. Holme and colleagues from various research institutions used TALEN- and CRISPR-Cas9-induced mutations to study the barley phytase gene HvPAPhy_a. The team aimed to validate the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as the importance of a promoter region of the gene containing GCN4, Skn1 and the RY elements, known to be involved in gene expression during grain filling.
The results confirm that the barley PAPhy_a enzyme is the main contributor to MGPA since the grains from the knock-out lines exhibited very low MGPA. Lines with mutations in the HvPAPhy_a promoter region GCN4/Skn1/RY also showed significantly reduced MGPA, proving its importance for HvPAPhy_a expression. Mutant grains with very low MPGA showed delayed germination as compared to grains of wild type barley.
Interestingly, lines with deletions located downstream of the promoter motif showed even lower MGPA levels, indicating that the GCN4/SKn1/RY motif is not the only element responsible for the level of PAPhy_a expression during grain maturation.
For more on this study, read the article in Plant Molecular Biology.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Kenya Approves Field Trials of Biotech Corn and Cotton
- Innate® 2nd Gen Potato Receives Government Clearance in Canada
- Genomic Study Shows Maize Adapted to Highlands 4,000 Years Ago
- PH Bureau of Plant Industry Invites Public Comments on Golden Rice
- Turkey Approves Four More Biotech Traits
- EFSA Releases Risk Assessment of New Sequencing Information on GM Soybean Events
- GM Plants Promise Fish Oils Aplenty
- Transcription Factor JUNGBRUNNEN1 Enhances Drought Tolerance in Tomato
- Overexpression of OsNRT2.3b Improves Phosphorus Uptake and Translocation in Rice
Plant Breeding Innovations
- Evaluation of HvPAPhy_a Gene in Barley Using CRISPR-Cas9 and TALENs
- CRISPR-Cas9-mediated Engineering of the γ-Aminobutyric Acid Pathway in Tomato
Beyond Crop Biotech
- Canadian Consumers First to Taste Commercialized GE Salmon
From the BICs
- SINO-Pak Hybrid Rice Research Center Inaugurated at ICCBS
- Science In Real Life: GMOs with the Van Eck Lab
- ISAAA Releases New Pocket K on CRISPR-Cas9
Subscribe to CBU: