Fat Content in Soybean Oil Modified with CRISPR-Cpf1February 22, 2017
A research team from the Center for Genome Engineering, within the Institute for Basic Research (IBS) in South Korea has successfully edited two genes that contribute to the fat content of soybean oil using the new CRISPR-Cpf1 technology. This technology is an alternative to the more widely used gene editing tool CRISPR-Cas9.
IBS scientists have previously used Cpf1 to edit human DNA cells. This time, they introduced the CRISPR-Cpf1 complex into plant cells. The team designed CRISPR-Cpf1 to cut two of the FAD2 genes in soybeans. These genes are part of the pathway that converts oleic acid into the polyunsaturated linoleic acid. By mutating FAD2 genes, the percentage of oleic acid in soybean seeds increases, resulting in healthier soybean oil.
The IBS research team also discovered at least three benefits of CRISPR-Cpf1 over CRISPR-Cas9: CRISPR-Cpf1 technique has shorter CRISPR-RNA (crRNAs), so the RNA can be synthesized chemically; CRISPR-Cpf1 creates larger deletions (7 base pairs) in the target gene, which is good for making the gene completely inoperative; and the type of cleavage left by Cpf1 might help further gene editing processes.
More details are available at the IBS News Center.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Climate-smart Rice Helps Farmers Face Climate Change
- Public learns about GMOs at ‘Harvest Money' Expo in Uganda
- Australian OGTR Approves Field Trial of GM Potato
- Downregulation of BnDA1 Improves Seed Weight and Organ Size in Oilseed Rape
- Overexpression of Wheat Gene TaOEP16-2-5B Enhances Heat and Drought Tolerance in Arabidopsis
Beyond Crop Biotech
- Penicillin Production in Penicillium chrysogenum is not Dependent on the Number of Synthesis Genes
From the BICs
- Helsinki and Cairo Universities Collaborate to Advance Science
- The GMO MOOC is Back!
- MSU Training Program on IPR and Technology Commercialization
- IPBO-VIB-UGent Course "Modern Breeding Techniques of Maize"
- New ISAAA Board Game: #BiotechisCool
- New Communication Materials from Cornell Alliance for Science
- Development of Targeted Mutant Rice using the CRISPR-Cpf1 System
- USPTO Releases Verdict on CRISPR Patent
- Fat Content in Soybean Oil Modified with CRISPR-Cpf1
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: