Development of Targeted Mutant Rice using the CRISPR-Cpf1 SystemFebruary 22, 2017
CRISPR-Cpf1 is a newly identified CRISPR-Cas system, and Cpf1 was recently used as a tool for targeted genome editing in mammalian cells. To test whether the engineered CRISPR-Cpf1 system could induce the production of rice mutants, Rongfang Xu, together with colleagues from Anhui Academy of Agricultural Sciences, selected two genome targets, the OsPDS and OsBEL genes.
After the transformation, the target region of OsPDS was examined, revealing that targeted mutagenesis could be achieved in rice using CRISPR-Cpf1. Analysis of the other target, OsBEL, confirmed that mutants were efficiently induced through transformation.
Further analysis also found that pre-crRNAs with a full-length direct repeat sequence exhibited higher efficiencies compared to mature crRNAs. These results indicate that the new CRISPR-Cpf1 can efficiently generate specific and heritable targeted mutations in rice.
For more information, read the article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Climate-smart Rice Helps Farmers Face Climate Change
- Public learns about GMOs at ‘Harvest Money' Expo in Uganda
- Australian OGTR Approves Field Trial of GM Potato
- Downregulation of BnDA1 Improves Seed Weight and Organ Size in Oilseed Rape
- Overexpression of Wheat Gene TaOEP16-2-5B Enhances Heat and Drought Tolerance in Arabidopsis
Plant Breeding Innovations
- Development of Targeted Mutant Rice using the CRISPR-Cpf1 System
- USPTO Releases Verdict on CRISPR Patent
- Fat Content in Soybean Oil Modified with CRISPR-Cpf1
Beyond Crop Biotech
- Penicillin Production in Penicillium chrysogenum is not Dependent on the Number of Synthesis Genes
From the BICs
- Helsinki and Cairo Universities Collaborate to Advance Science
- The GMO MOOC is Back!
- MSU Training Program on IPR and Technology Commercialization
- IPBO-VIB-UGent Course "Modern Breeding Techniques of Maize"
- New ISAAA Board Game: #BiotechisCool
- New Communication Materials from Cornell Alliance for Science
Read the latest:
- Crop Biotech Update (October 5, 2022)
- Genome Editing Supplement (October 5, 2022)
- Gene Drive Supplement (September 28, 2022)
Subscribe to CBU: