Gene Replacements and Insertions in Rice via Intron-Targeting CRISPROctober 5, 2016
Sequence-specific nucleases have been exploited to create targeted gene knockouts in various plants. However, replacing a fragment and even obtaining gene insertions at specific loci in plant genomes remain challenging. Chinese Academy of Sciences' Jun Li, along with a group of researchers, describes an efficient intron-mediated site-specific gene replacement and insertion that generate mutations using non-homologous end joining (NHEJ) using CRISPR/Cas9.
Using a pair of single guide RNAs (sgRNAs) targeting adjacent introns and a donor DNA template including the same pair of sgRNA sites, the team achieved gene replacements in the rice gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) at a frequency of 2.0%. The team also obtained targeted gene insertions at a frequency of 2.2% using a sgRNA targeting one intron and a donor DNA template including the same sgRNA site.
Rice plants harboring the OsEPSPS gene with the intended substitutions were also found to be glyphosate-resistant. Furthermore, the site-specific gene replacements and insertions were inherited by the next generation. These new approach can be used to replace targeted gene fragments and insert DNA sequences into specific genomic sites in rice and other plants.
For more information, read the article in Nature Plants.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO: Transform Agriculture to Address Global Challenges
- Uganda Holds Banana Research Training for African Scientists and Biotechnology Regulators
- Researchers Modify Yeast to Show How Plants Respond to Auxin
- US Congress Ratifies Historic Global Food Security Treaty
- GM Maize MIR162 Harvested in Large Scale Field Trial in Vinh Phuc, Vietnam
- Conference Tackles Legal Obligations and Compensation on Biosafety Regulations in Vietnam
- Iloilo Stakeholders Informed about New Biosafety Regulations in PH
- Seminar at EU Parliament Tackles GM Crops Concerns
- Bt Maize Has No Effect on Population Dynamics of an Endophytic Bacterial Community
- OsLPR Genes Play Roles in Maintaining Phosphate Homeostasis in Rice
Plant Breeding Innovations
- Germ-line-Specific CRISPR-Cas9 Systems Improve the Production of Heritable Gene Mutations in Arabidopsis
- Gene Replacements and Insertions in Rice via Intron-Targeting CRISPR
Beyond Crop Biotech
- Scientists Sequence Complete Fruit Fly Genome
- 2016 Nobel Prize in Physiology or Medicine Goes to Autophagy Researcher
- Infographic: How GMOs and Sustainable Farming Practices Can Improve Air Quality
Read the latest:
- Crop Biotech Update (September 21, 2022)
- Genome Editing Supplement (September 21, 2022)
- Gene Drive Supplement (August 31, 2022)
Subscribe to CBU: