Germ-line-Specific CRISPR-Cas9 Systems Improve the Production of Heritable Gene Mutations in ArabidopsisOctober 5, 2016
CRISPR/Cas9 is widely used for targeted gene modifications in plants. The system has two components, a single-guide RNA (sgRNA) for target DNA recognition and the CRISPR-associated protein 9 (Cas9) for DNA cleavage. However, while ubiquitously expressed CRISPR/Cas9 systems (UC) generate targeted gene modifications with high efficiency, only those produced in reproductive cells are passed on to the next generation.
Yanfei Mao of the Chinese Academy of Sciences, together with a team of researchers, reports the design and characterization of a germ-line-specific Cas9 system (GSC) for Arabidopsis gene modification in male gametocytes.
Two genes were targeted by both systems, UC and GSC, for analysis. Mutations generated by the GSC system were rare in T1 plants, but were abundant in the T2 generation. Majority of the T2 mutant population generated using the UC system were chimeras while only 29% of those produced via GSC were chimeras.
Further analysis of the T2 population showed that the heritable gene mutations were 37% higher in the GSC system compared to the UC system.
For more on this study, read the article in Plant Biotechnology Journal.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO: Transform Agriculture to Address Global Challenges
- Uganda Holds Banana Research Training for African Scientists and Biotechnology Regulators
- Researchers Modify Yeast to Show How Plants Respond to Auxin
- US Congress Ratifies Historic Global Food Security Treaty
- GM Maize MIR162 Harvested in Large Scale Field Trial in Vinh Phuc, Vietnam
- Conference Tackles Legal Obligations and Compensation on Biosafety Regulations in Vietnam
- Iloilo Stakeholders Informed about New Biosafety Regulations in PH
- Seminar at EU Parliament Tackles GM Crops Concerns
- Bt Maize Has No Effect on Population Dynamics of an Endophytic Bacterial Community
- OsLPR Genes Play Roles in Maintaining Phosphate Homeostasis in Rice
Beyond Crop Biotech
- Scientists Sequence Complete Fruit Fly Genome
- 2016 Nobel Prize in Physiology or Medicine Goes to Autophagy Researcher
- Infographic: How GMOs and Sustainable Farming Practices Can Improve Air Quality
- Germ-line-Specific CRISPR-Cas9 Systems Improve the Production of Heritable Gene Mutations in Arabidopsis
- Gene Replacements and Insertions in Rice via Intron-Targeting CRISPR
Read the latest:
- Biotech Updates (May 31, 2023)
- Gene Editing Supplement (May 31, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: