CIP Develops Marker-free PLRV Resistant Potato
August 24, 2016| |
Potato leaf roll virus (PLRV) is one of the most prevalent pathogens of potato, causing serious economic yield losses globally. Producers control PLRV by using certified seeds and by applying insecticides. However, these practices are costly and such products are not always available to small-scale farmers. Thus, scientists from the International Potato Center (CIP) developed marker-free transgenic potato plants with intense resistance to PLRV.
The researchers created an inverted repeat construct corresponding to a portion of the PLRV coat protein gene under the control of a constitutive promoter. Then they transferred it into a transformation vector with Cre-loxP system to cut the nptII antibiotic resistance marker gene. The team generated a total of 58 transgenic events, with seven events showing high resistance. Of the seven transgenic events, four events showed extremely high resistance, a degree which has not been reported in previous studies. Further analysis showed the correlation of the PLRV silencing RNA accumulation and the level of resistance to PLRV.
Read the research article in Transgenic Research.
| |
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Africa's Biosafety Agencies Seek to Harmonize Approaches to Regulatory Systems
- African Countries Strive to Meet Int'l Biosafety Obligations
- Photosynthesis Discovery in Wheat to Yield Faster Growing Crops
- Australia's OGTR Receives Application for Field Trials of Biotech Cotton
- Murdoch University Researchers Increase Wheat Protein to More than 14%
- European Seed Association Asks EU to Recognize Role of Plant Breeding in Addressing Societal Challenges
- EFSA Publishes Public Opinion for Market Release of Pioneer GM Soybean
-
Research Highlights
- CIP Develops Marker-free PLRV Resistant Potato
- Researchers Map QTL for Dwarf Architecture in Oilseed Rape
-
Beyond Crop Biotech
- Identification of Candidate Genes for Calanolide Synthesis in Santa Maria Tree
-
Announcements
- MOOC: Science and Politics of the GMO
- IUMS Congresses 2017
-
Resources
- Biotech Country Facts and Trends
-
Plant
- Swiss Academies: Strict Regulations Not Necessary for NBTs
- Broad Virus Resistance in Cucumber Developed using CRISPR
-
Read the latest: - Biotech Updates (December 11, 2024)
- Gene Editing Supplement (December 11, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet