Salk Institute Scientists Map Landscape of Genetic and Epigenetic Regulation in PlantsMay 25, 2016
A new technique developed by scientists from Salk Institute to rapidly map regions of DNA targeted by regulatory proteins could provide insight into what makes some plants drought tolerant or disease resistant. Revealing this landscape of protein-binding zones on DNA, called the "cistrome," shows how plants control where and when genes are expressed. Previous methods to map the cistrome in plant cells were difficult and slow, but the new approach offers a sweeping view of this critical aspect of genetic regulation.
The cistrome was mapped using a system that the researchers created, where they could add a tagged transcription factor to a library of DNA, let it bind and then isolate all the DNA-protein pairs. The method, called DNA affinity purification sequencing (DAP-seq), vastly expands how much more information scientists can understand about transcription factors and their binding sites. To test DAP-seq, the researchers mapped 529 transcription factors bound to the genome of Arabidopsis thaliana and identified 2.7 million binding sites.
They repeated the experiments using DNA containing or not containing cytosine methylation, and the binding patterns of about three-quarters of the transcription factors that they tested changed. The results show not only how regulatory proteins alter gene expression, but also the roles the epigenomic methylation marks may play in this regulation.
For more details, read the news release at the Salk Institute website.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Scientists Investigate GM Food Awareness in Enugu, Nigeria
- Malawi's Seed Sector Calls on Govt to Adopt GM Crops
- Swaziland Agri Director Expresses Support to Amend the Biosafety Law
- Chance Finding in Guelph Study Could Transform Future of Plant Production
- Salk Institute Scientists Map Landscape of Genetic and Epigenetic Regulation in Plants
- University Illinois Scientist Identifies Mechanism for Herbicide Resistance in Palmer Amaranth
- Biologists Discover How Plants Reconstitute Stem Cells
- Philippine American Academy of Science and Engineering Calls for Resumption of Bt Eggplant Research
- The Royal Society Releases Guide on GM Plants
- Scientists Battle to Stem Onslaught of Pseudoscience in Europe
- British Crop Protection Council Challenges Green Alliance's Opposition to Glyphosate and GM Crops
- WRKY Genes from Wheat Confer Drought and Heat Resistance in Arabidopsis
- GmSAMT1 Overexpression in Soybean Confers Resistance to Soybean Cyst Nematode Races
Beyond Crop Biotech
- Researchers Develop Cell Labeling via Photobleaching Tagging Method
- Soluble Expression of Spike Protein of Porcine Epidemic Diarrhea Virus in E. coli
Read the latest:
- Biotech Updates (February 1, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to BU: