Functional Human Growth Factors Produced from an Insect-Baculovirus SystemJune 11, 2014
Growth factors (GFs) are naturally signalling proteins that bind to specific receptors on the cell surface. Progress has been made in identifying these GFs and understanding their pathways. The wider clinical and commercial application of these factors now relies on their scalable cost-efficient production as bioactive molecules.
The ability of the insect larvae Trichoplusia ni as living bioreactors, in combination with the baculovirus vector expression system, in producing three fully functional human GFs was evaluated. The three human growth factors to be produced were: the human epidermal growth factor (huEGF), the human fibroblast growth factor 2 (huFGF2) and the human keratinocyte growth factor 1 (huKGF1).The expression levels of the three growth factors obtained per gram of insect biomass were of 9.1 mg huEGF, 2.6 mg huFGF2 and 3 mg huKGF1, respectively.
Different modifications of the insect-baculovirus system were also used in attempts to optimize production of the growth factors. The insect-derived recombinant GFs were then characterized in terms of biological activity.
This discovery opens the possibility of a cost-efficient and scalable production of these highly valuable molecules in a system that favors its wide use in therapeutic or cosmetic applications.
To read more on this study, feel free to visit: http://www.sciencedirect.com/science/article/pii/S0168165614002764.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- International Organizations Reconfirm Support for Biotech Wheat Commercialization
- Scientists Analyze Citrus Genomes to Produce Resistant Varieties
- Kenyan Governors Call for Lifting of GMO Ban
- Phaseolus Genome Gives Insights into Nitrogen Fixation
- Scientists Show How Bacterial Protein in Aphid Saliva Triggers Plant Defense
- New Website for Fruit Genome Databases
- CCAFS-SEA and Partners Discuss Effective Communication on Climate Change
- A Comparative Study of GMO Labeling in the South Korea, US, and EU
- Australian GM Farmer Wins Landmark Case
- Scientists Uncover Root Mechanism to Boost Crop Performance
- Effect of Excessive UV-B Light on Soybean Plants
- OsMYB103L Regulates Cellulose Synthase Genes and Improves Leaf Shape and Strength in Rice
- Potassium Transport Gene in Soybean Enhances Its Resistance to Soybean Mosaic Virus
- Impact of Virus Resistant GM Wheat on Microbial Community Diversity
Beyond Crop Biotech
- Human Genome Project Gets Protein Equivalent
- Sheep Genome Decoded
- Functional Human Growth Factors Produced from an Insect-Baculovirus System
- International Conference on Asian Food Security
- ISAAA Infomercial: What Do People Say About Biotech?
- Biotech Corn Farmer Story in ISAAA Blog
Read the latest:
- Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
Subscribe to CBU: