Crop Biotech Update

Accumulation of Recombinant Human Coagulation Factor IX in Transgenic Soybean Seeds

November 26, 2010

Soybean is one of the practical options for producing recombinant proteins essential for human blood coagulation because its "high protein content, known regulatory sequences, efficient gene transfer protocols, and scalable production system under greenhouse conditions." Nicolau Cunha of the University of Brasilia, together with other scientists, reports the expression and stable accumulation of human coagulation factor IX (hFIX) in genetically engineered soybean seeds, published in the December 2010 issue of the Transgenic Research journal.

Using a gene gun, they introduced a plasmid with hFIX gene in the soybean embryonic axes obtained from mature seeds. The hFIX protein was expressed in protein storage vacuoles and composed about 23 percent of the total soluble protein content of the transgenic seeds. Protein extracts from transgenic seeds showed a blood-clotting activity of up to 1.4 percent of normal plasma.

Read the abstract at http://www.springerlink.com/content/h3n5178t0143737k/.