CRISPR-dMac3-Cas9 System Used to Investigate Potato Tuber Starch TraitSeptember 22, 2021
Tokyo University of Science researchers explored the potato tuber starch trait by developing potato mutants lacking a starch branching enzyme gene using CRISPR-Cas9 attached to the translation enhancer dMac3. Their findings are published in J-stage Plant Biotechnology journal.
The potato tuber starch trait can be altered based on the composition of amylose and amylopectin. The amount of amylopectin depends on the activity of starch branching enzymes, and one of which is abundant in tubers is SBE3. Thus, the researchers developed SBE3 mutants using the CRISPR-dMac3-Cas9 system.
This led to mutations in the SBE3 in 89 of 126 potato plants. Of the 89 mutants, 10 lines had four mutant SBE3 genes, showing an 8% efficiency of target mutagenesis attained. Further analysis confirmed that the transformants are loss-of-function mutants of SBE3. The mutants also exhibited normal growth, similar to the wild-type plant, and yielded sufficient amounts of tubers.
Read more results in J-stage Plant Biotechnology.
You might also like:
- Plant Breeding Innovation: CRISPR-Cas9
- Scientists Develop Transgenic Potato with Enhanced Tuber Quality and Stress Tolerance
- Scientists Discover RNA Controlling Tuber Formation in Potatoes
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Asian Regional Workshop on Current and Upcoming Items under the CBD and its Protocols
- Biotech Stakeholders Gather to Celebrate Africa Biotechnology Gains at ABBC2021
- Statements by Known Organizations Influence Consumer's Attitude about GMOs
- US Scientists Embark on Developing Edible Plant-based Vaccines
- Australian OGTR Approves Commercial Release of GM Canola
- Australian Researchers Discover Barley Powdery Mildew Resistance Genes
- John Innes Centre Research Group Identifies Microbial Molecule that Turns Plants into Zombies
- French Researchers and Experts Appeal to President Macron to Support Plant Biotechnologies
- Scientists Assess Impact of Beta-carotene-enriched Soybeans on Soil Microorganisms
- CRISPR-dMac3-Cas9 System Used to Investigate Potato Tuber Starch Trait
- Researchers Identify Abiotic Stress Tolerance Genes for Editing in Upland Rice
- Japan Starts Sale of Genome-Edited High-GABA Tomato
Read the latest:
- Biotech Updates (November 22, 2023)
- Gene Editing Supplement (November 15, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: