Protocol Reveals How to Generate Clonal Seeds from Hybrid Rice with CRISPR-Cas9August 4, 2021
Chinese Academy of Agricultural Sciences researchers outline the methods for generating clonal seeds from hybrid rice with CRISPR-Cas9 in the book titled CRISPR-Cas Methods.
The use of heterosis in rice production has significantly improved yield. However, hybrid vigor cannot be preserved in the offspring because of genetic segregation. Simultaneous editing of REC8, PAIR1, and OSD1 genes turns meiosis into mitosis and eventually produces clonal gametes, while knockout of the MTL gene leads to maternal haploid seeds development. Genome editing of all these four genes in hybrid rice simultaneously could fix the heterozygosity and obtain clonal seeds from hybrid rice. The protocol provides a detailed technique for generating clonal seeds from hybrid rice by using the multiplex CRISPR-Cas9 technology.
Read the protocol in Springerlink.
You might also like:
- Pocket K No. 54: Plant Breeding Innovation: CRISPR-Cas9
- How about CRISPR-edited Crops in Organic Agriculture?
- Researchers Control Rice Hull Color via CRISPR
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Transgenic Maize Trials Show Outstanding Results in Nigeria
- Experts Propose Measures to Achieve Full Potential of Agricultural Biotechnology in Africa
- Study Documents 13 Years of Adoption and Learning by US Soybean Farmers
- Penn State Researchers Identify Gene that Regulates Angle of Root Growth in Corn
- Insights on Flowering from Two Studies Could Boost Cassava Production
- Genome-Wide Association Study Identifies Genes for Wheat Resistance to Tan Spot
- US Plant Scientists Oppose Ordinance Prohibiting Planting GMOs, Cite Their Benefits Instead
- Scientists Release Genome Assembly for Fielder Wheat Cultivar
- Research Reveals Secrets of an "Immortal" Plant
- Study Explores Degradation of Cry Proteins After Harvest
- Protocol Reveals How to Generate Clonal Seeds from Hybrid Rice with CRISPR-Cas9
- Experts Modify RNA Guides for CRISPR Tools and Therapies
Read the latest:
- Biotech Updates (February 21, 2024)
- Gene Editing Supplement (February 14, 2024)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: