DNA-Free Screening System for Gene Editing in Hot and Sweet Bell PeppersOctober 7, 2020
Soil-grown leaf- or callus-derived pepper protoplasts are a useful system in hot and sweet bell peppers for the screening of efficient guide RNAs for CRISPR-Cas9 or CRISPR-Cas12a (Cpf1). This breakthrough was reported by researchers from Kangwon National University, South Korea, and published in BMC Plant Biology.
DNA-free, CRISPR-based genome editing is a simple and promising technique used in improving crops. The efficacy of designed CRISPR-based genome editing tools is vital for successful precision gene editing of crops. Thus, the study revealed an efficient system for screening of efficient guide RNAs for CRISPR-Cas9 or CRISPR-Cas12a (Cpf1). CRISPR-Cas9 or Cpf1 were delivered as CRISPR-RNP complexes of purified endonucleases mixed with the designed single guide RNA, which can edit the target gene, CaMLO2 in two pepper cultivars with whole-genome sequenced. The designed guide RNAs are conserved for CaMLO2 in both hot and sweet peppers and cleave CaMLO2 in vitro. CRISPR-Cas9- or -Cpf1-RNP complexes were transfected into purely isolated protoplasts of the hot pepper and sweet pepper. Further analysis revealed that the targeted CaMLO2 gene was differentially edited in both cultivars, depending on the applied CRISPR/RNPs.
Read more findings from the open-access research article.
You might also like:
- Sweet pepper (Capsicum annuum) GM Event
- Scientists Release Most Complete Genetic Map of Spanish Peppers
- Two Methods Used in Finding Capsaicinoid Candidate Genes in Hot Pepper
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Researchers Sequence Genomes of 600 Green Millet Plants, Find Gene for Dispersal
- Paraguay Simplifies Procedures in the Approval of GE Crops
- Transgenic Hybrid Corn Harvest Boosts Cuba's Confidence in Corn Yield
- FSANZ Seeks Public Comment for GM Corn Approval
- Farmer Survey Reveals Significant Economic, Environmental Benefits From GM Corn in Vietnam
- Research Explains Farmer Reluctance to Adopt New Technologies
- Gene for Resistance to Devastating Tomato Virus Now Identified
- Meta-analysis Detect Candidate Rice Genes Involved in Salt Tolerance
- DNA-Free Screening System for Gene Editing in Hot and Sweet Bell Peppers
- Scientists Explore on Genetic Transformation of Mosquitoes
- Researchers Develop Pale Purplish-Pink Petunia via CRISPR-Cas9
- Researchers Engineer Drug-Like Compounds that Disable SARS-CoV-2's Replication Engine
Read the latest:
- Biotech Updates (October 4, 2023)
- Gene Editing Supplement (September 27, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: