Researchers Engineer a Rapid TALEN Assembly Protocol for Genome EditingSeptember 30, 2020
A speedy transcription activator-like effector nuclease (TALEN) preparation protocol was developed based on widely used Golden Gate TALEN and TAL Effector Kit 2.0. This new protocol can be used to prepare any custom 18-bp binding TALENs in about 12 hours, which is faster than CRISPR. The results of the study are published in ACS Publications.
According to the research article, the protocol involves the use of a set linear monomers, a final TALE-FokI backbone plasmid, and a pipeline to assemble the ready-to-use TALEN expression plasmid, which were all designed for the study. The TALEN construction pipeline can simply acquire several positive colonies with high efficiency of over 80 percent. Results of the tests involving five NF-κB genes showed that the new protocol has high efficiency, reproducibility, reliability, and applicability. Furthermore, it was shown that the fabricated TALEN has better editing efficiency than CRISPR.
Get more details about the protocol in ACS Publications.
You might also like:
- Scientists Use TALENs for Targeted Genome Editing of Potato
- Genome Editing in Potato using TALENs Delivered via Agroinfiltration
- New Bicistronic TALENs Enhance Genome Editing
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Animal Biotech Online Discourses Cover Genome Editing Regulatory Approaches
- Science and Scientists Held in High Esteem Across the World
- Review Says Farmer Knowledge Key to Finding More Resilient Crops
- Myanmar Scientists, Academics Step Up for Biotechnology Information Portal Launch
- Research Team to Genetically Improve Millet for Large-Scale Agriculture in Saudi Arabia
- Researchers Find Way to Protect Plants from Fungal Infection
- Researchers Overexpressed Transcription Factors to Improve Cotton Lint Yield
- Detection of Off-Target Effect in Genome Editing by Integration-Defective Lentiviral Vector
- Gene Editing of SlLBD40 Improves Drought Tolerance in Tomato
- Researchers Engineer a Rapid TALEN Assembly Protocol for Genome Editing
- Sewage Testing Shows Prevalence of COVID-19 Infection
- Farmers Highly Vulnerable to COVID-19, Says Study
Read the latest:
- Biotech Updates (September 27, 2023)
- Gene Editing Supplement (September 27, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: