
Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in Rice
November 27, 2019A procedure to develop new Jasmonate (JA)-deficient mutant using CRISPR-Cas9 in rice was presented by the researchers from Universite de Montpellier, France and partners in Springer Protocols. They targeted the Allene Oxide Cyclase (OsAOC) gene because it is a single copy gene in the JA biosynthesis pathway in rice.
The role of JA in various plant physiological processes and its interactions with the environment has been well-documented. Mutants with dysfunctional JA production and/or signaling are useful in investigating the function of JA, which is a phytohormone. However, previous studies presented mutants that were mostly generated using random mutagenesis. In the recent study, CRISPR-Cas9 was employed for a more precise genome editing at specific loci while limiting unwanted mutations.
The protocol presented describes the selection of the 20-nt target sequence, construction of the binary vector, and strategy for selecting the T-DNA-free mutant.
Read more from the book Methods in Molecular Biology.
You might also like:
- New Research Reveals Atomic Level Defense Secrets of Plants
- International Research Team Finds Novel Function of Plant Hormone Jasmonic Acid
- Scientists Find a Gene for Boosting Plant Pest Resistance
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
COVID-19 Alerts
- Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in Rice
- Study Pinpoints an NLR Gene that Confers Partial Resistance to Rice Blast Fungus
-
News from Around the World
- Science and She: Empowering Women in Science
- Scientists Develop "War Room" Simulations to Fight Crop Diseases
- Gene-editing Increases Sorghum's Protein Content
- PH Scientists Lead Discussions on Biotech Crops Safety with Food and Feed Industry
- Biotech Saves Lives, DOH Undersecretary Says
- Engineered Bacteria Produces BeeFree Honey
-
Research Highlights
- Research Team Discovers Gene for Iron Control in Plants
- Utrecht Biologists Discover Mechanism for Plant Growth Under High Temperatures
-
Plant Breeding Innovations
- Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in Rice
- Study Pinpoints an NLR Gene that Confers Partial Resistance to Rice Blast Fungus
-
Read the latest: - Crop Biotech Update (January 25, 2023)
- Genome Editing Supplement (January 18, 2023)
- Gene Drive Supplement (January 25, 2023)
-
Subscribe to CBU: - Share
- Tweet