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Crop Biotech Update

Transformation of E. coli Modified gdha Gene into Tobacco Improves Productivity and Stress Tolerance

June 26, 2019

The bacterial gdhA gene encodes NADH-GDH enzyme. The gdhA-transgenic tobacco (Nicotiana tabacum) plants exhibited high growth performance and enhanced herbicide resistance as well as drought tolerance. In addition, the gdhA gene when expressed in maize plants could increase productivity because of improving stress tolerance. 

In a study published in Academia Journal of Biology, the researchers isolated and analyzed a gdhA gene from the E. coli strain JM109. Then, the E.coli derived-gdhA sequence was modified by using OptimumGeneâ„¢ software for plant compatibility. The optimized gdhA gene was introduced into the A. tumefaciens strain EHA105. In order to examine the expression of the gdhA gene, gdhA-transgenic plants were generated via Agrobacterium-mediated transformation into tobacco K326. Further analysis showed that two out of 20 plants were positive to the presence of the gdhA gene.  These results suggested that the gdhA expression construction can be transformed into other crops such as maize.

For more information, read the full article in Vietnamese at Academia Journal of Biology.