Scientists Report First Genetic Transformation of EinkornFebruary 13, 2019
Scientists from All-Russia Research Institute of Agricultural Biotechnology in Moscow reported the first genetically modified einkorn produced. Domesticated einkorn (Triticum monococcum) is one of the oldest cultivated cereal crops globally, and its genetic characteristics (small genome size, low ploidy, high genetic polymorphism) make it a good model for studying the genomics and proteomics of Triticeae.
Successful genetic modification of einkorn was achieved using a gene gun. Young tissues of spring einkorn were bombarded with a plasmid containing the reporter gene GFP (green fluorescent protein) and the selectable bar gene (bialaphos resistance gene). Gas pressure, microcarrier size and developmental stage of target tissue were adjusted to attain stable transformation.
Results showed that the bombarded einkorn tissues were recalcitrant to regeneration, but certain modifications of the culture medium have been shown to increase the production of transgenic plants. In various experiments, independent transgenic plants were produced at frequencies of up to 0.6%. Molecular analysis, marker gene expression and herbicide treatment demonstrated that gfp/bar genes were stably integrated into the einkorn genome and successfully inherited over several generations. The transgenes, as dominant loci, segregated in both Mendelian and non-Mendelian fashion due to multiple insertions. Fertile homozygous T1-T2 populations of transgenic einkorn that are resistant to herbicides were selected.
The results of the study could be a starting point for the application of transgenesis and genome editing and advance comparative functional genomics in einkorn and other cereals.
Read more from BMC Biotechnology.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- A Comprehensive Review of the Environmental Safety of Bt Crops Now Published
- New Chocolate has Its Own Pro-GMO Label to Promote GMO Farming
- NASA: Tree Planting and Agri in China and India Make Earth Greener
- Experts Call for Urgent Political Support for Agri-biotech in the Philippines
- Genes that Resist White Rust Pathogen Found
- Study Finds Attitudes of the Youth toward GM Food Affected by Family Members
- Rothamsted Research Applies for New Trials of GM Camelina
- COGEM: No Risk in Import and Processing of GM Maize Bt11
- Scientists Report First Genetic Transformation of Einkorn
- Haplotype Analysis of Rice Genes Helps Development of Tailor-made Rice with Improved Genetic Gains
- GMOs Not the Cause of Monarch Butterfly Decline, Study
Beyond Crop Biotech
- NRGene and Kayagene Introduce the First Fully-Phased Cannabis Genome
- International Conference on Industrial Crops
- CRISPR-Cas9 Used for Targeted Disruption of FAD2–2 Microsomal Omega-6 Desaturase in Soybean
- Improved CRISPR System with Power on Switch for Gene Editing
Read the latest:
- Biotech Updates (February 21, 2024)
- Gene Editing Supplement (February 14, 2024)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: