Screening for CRISPR and TALENs Edits, Better with Ribonucleoproteins
November 28, 2018 |
Researchers develop ribonucleoprotein (RNP)-based screening for genome modifications by CRISPR and TALENs because of limitations of existing methodologies for screening. These methods include polymerase chain reaction (PCR) with restriction enzyme digestion, T7EI cleavage assay, and sequencing, which are limited by sequence requirement, non-applicability in high ploidy species, non-detection of heterozygous mutations, and high cost.
PCR/RNP works by cleaving DNA sequences that are identical to the RNA in the RNP complex. The expected result of using this method is multiple bands for non-edited plants and intact single bands for edited plants for diploid or polyploid plants. Researcher Zhen Liang from University of Chinese Academy of Sciences and colleagues tested this method in hexaploid wheat and diploid rice that were edited using CRISPR and TALENs. Results showed higher sensitivity of the method than DNA sequencing, high applicability in hexaploid wheat, and ability to detect edited genes using TALENs. This method proved to be useful because of its low cost and short time span requirement.
For more information, read the article in Plant Biotechnology Journal.
|
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Biotech Law Will Be Passed Soon in Uganda, S&T Chairperson Assures Farmers
- Study Shows Plant Characteristics Shaped by Parental Conflict
- Citizen Science Can Play A Role in Addressing Agricultural Challenges
- Argentina One Step Away from GM Wheat Commercialization
- Men More Positive about GM Foods, Survey Says
- Australian OGTR Approves Field Trial of GM Canola
- Study Shows Positive Impact of Bt Cotton Adoption Among Farmers in Pakistan
- Nobel Laureate Rallies Global Support in Favor of GMOs
- Report: Pakistan Achieves Biotech All-Time High in 2017
-
Research Highlights
- Gene Improves Heat and Drought Tolerance in Wheat and Arabidopsis
- Cotton Gene Enhances Brown Fiber Quality
- Development of 3rd Generation Climate Resilient and Dry Direct Seeded Rice Varieties
-
Beyond Crop Biotech
- Research Reveals How Ancient Viruses Got Cannabis High
-
Resources
- Map Shows Public Sector Biotech Products in the Pipeline Around the World
-
Plant
- CRISPR-Cas9 Delivered in Wheat Using Agrobacterium
- Screening for CRISPR and TALENs Edits, Better with Ribonucleoproteins
- CRISPR-Cas12a Applied in Rice
- Researchers Use CRISPR-Cas9 to Target Ripening Genes in Tomato
-
Read the latest: - Biotech Updates (November 6, 2024)
- Gene Editing Supplement (November 13, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet