CRISPR-Cas9 Delivered in Wheat Using AgrobacteriumNovember 28, 2018
Researchers have been using biolistics in delivering the genome editing complex in wheat due to difficulties in Agrobacterium-mediated genetic transformation in the species. However, scientist Shujuan Zhang from Shandong Academy of Agricultural Sciences in China and colleagues overcome this limitation by successfully targeting three wheat genes using CRISPR-Cas9 delivered via Agrobacterium-mediated genetic transformation.
Agrobacterium-mediated genetic transformation is considered to be better than biolistic delivery because the latter produces single and more specific insertions of the genome editing complex in the genome and does not require expensive equipment. In the study, seven target sites in genes Pinb, waxy and DA1 were selected for gene editing. Mutations were detected through Illumina sequencing. Results showed efficient gene editing in the target, with 54.17 percent mutation efficiency. Mutations are present in the A and B genomes but not in the D genome. Off-target mutations were not detected in the mutant plants. These results show the applicability of Agrobacterium-mediated genetic transformation in wheat genome editing and genetic improvement.
For more information, read the article in BMC Plant Biology.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Biotech Law Will Be Passed Soon in Uganda, S&T Chairperson Assures Farmers
- Study Shows Plant Characteristics Shaped by Parental Conflict
- Citizen Science Can Play A Role in Addressing Agricultural Challenges
- Argentina One Step Away from GM Wheat Commercialization
- Men More Positive about GM Foods, Survey Says
- Australian OGTR Approves Field Trial of GM Canola
- Study Shows Positive Impact of Bt Cotton Adoption Among Farmers in Pakistan
- Nobel Laureate Rallies Global Support in Favor of GMOs
- Report: Pakistan Achieves Biotech All-Time High in 2017
- Gene Improves Heat and Drought Tolerance in Wheat and Arabidopsis
- Cotton Gene Enhances Brown Fiber Quality
- Development of 3rd Generation Climate Resilient and Dry Direct Seeded Rice Varieties
Beyond Crop Biotech
- Research Reveals How Ancient Viruses Got Cannabis High
- Map Shows Public Sector Biotech Products in the Pipeline Around the World
- CRISPR-Cas9 Delivered in Wheat Using Agrobacterium
- Screening for CRISPR and TALENs Edits, Better with Ribonucleoproteins
- CRISPR-Cas12a Applied in Rice
- Researchers Use CRISPR-Cas9 to Target Ripening Genes in Tomato
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: