
CRISPR-Cas9 Applied to Identify ToMV Defense Gene in Tomato
September 26, 2018The researchers mutate DCL2b and find 5 bp homozygous deletions that caused a premature stop codon in the mutants. The mutants have normal developmental morphology, but show severe defects in the leaves, flowers, and fruits when infected with Tomato Mosaic Virus (ToMV). This result indicates the major role of DCL2b in the plant's defense against ToMV. Further analyses show the role of the protein in the biogenesis of 22-nt miRNAs. Overall, these results provide new insights in tomato viral defense.
For more information, read the article in Horticulture Research.
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