CRISPR Nucleases Evaluated for Genome Editing in MaizeAugust 1, 2018
CRISPR-Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. Iowa State University researchers compared the activities of these two nucleases by targeting the maize glossy2 gene coding region. The research team, led by Keunsub Lee, introduced the SpCas9‐guide RNA (gRNA) and LbCas12a‐CRISPR RNA (crRNA) into maize inbred embryos using Agrobacterium‐mediated transformation.
Analysis showed that 90-100% of the Cas9‐edited T0 plants carried mutations, 63-77% of which were homozygous or biallelic mutants. Meanwhile, 0-60% of Cas12a-edited T0 plants had the on-target mutations. Analysis of potential off-target sites for the two nucleases identified 18 and 67 potential off‐targets for SpCas9 and LbCas12a, respectively. Further analysis of the off‐target sites revealed no detectable mutations in the T1 plants.
These results suggest that the CRISPR-Cas9 system used in this study is highly efficient and specific for genome editing in maize. On the other hand, CRISPR-Cas12a needs to be improved for genome editing.
For more information, read the article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Genetically Modified Rice Can Neutralize HIV
- Biotech Discussed at National Plant Variety Protection Dialogue in Uganda
- Nigeria Registers Biotech Cotton Varieties for Access to Farmers
- USDA-APHIS Releases Assessment Documents of GE Low Gossypol Cotton for Public Comment
- AgResearch Tests GM High Metabolizable Energy Ryegrass
- Experts Review Social and Economic Impacts of Biofortification through Biotech
- Researchers Discover How Plants Respond to Attacks
- Rice with Less Stomata Needs Less Water; Better Suited for Climate Change
- Researchers Find Gene Related to High-Oil Content in Oil Palm
- Differences in Coffee Quality may be Explained by the Gene Expressions in Coffee Bean During Ripening
- Co-Expression of ApGSMT2g and ApDMT2g Enhances Salt Tolerance in Cotton
Plant Breeding Innovations
- CRISPR Nucleases Evaluated for Genome Editing in Maize
- Researchers Discover Regulator of Endosperm Development in Rice
- Epigenetic Basis of High Regeneration Ability of an Elite Cotton Genotype Jin668
Beyond Crop Biotech
- University of Adelaide Researchers Uncover Barley's Brewing Secrets
- C4 Photosynthesis Gene Discovered in Foxtail Millet
- Transcription Factor Genes Increase Biomass Production in Switchgrass
From the BICs
- PABIC Organizes SciCom and Policy Making Lecture
Subscribe to CBU: