Application of CRISPR-Cas9 in China's Medicinal YamJuly 4, 2018
Dioscorea zingiberensis, a type of yam in China, is a major pharmaceutical plant that produces diosgenin, a raw material for steroidal hormones. To date, there is no genome editing approach reported for D. zingiberensis. The CRISPR-Cas9 system proved to be an efficient targeted genome modification tool and has been successfully applied in many plants. The team of Shan Feng from Wuhan University in China, aimed to apply the CRISPR-Cas9 system in D. zingiberensis.
The team designed a guide RNA to target the farnesyl pyrophosphate synthase gene (Dzfps), which is a critical gene involved in the synthesis of secondary metabolites. High frequencies of mutants were detected in T0 plants. Among the transformed plants, nine mutants that contained mutations at the predicted site were identified. The transcript levels of Dzfps, was significantly decreased, leading to a decrease in the content of squalene in the isolated mutants compared to wildtype plants.
The study revealed that CRISPR-Cas9 can be an effective approach for genome modification in D. zingiberensis.
For more information, read the article in Plant Cell, Tissue and Organ Culture.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Study Shows Labeling Reduced GMO Food Fears
- GM Tomatoes Give Fish a Pinkish Glow
- Biotech Corn Variety Shows Resistance to Ear Rot
- ISAAA Reports New Record-High Adoption of GM Crops
- Australian OGTR Approves Commercial Release of GM Safflower
- New Mechanism for Plant Hormone Auxin Discovered
- "GMOs Are Vital Against Hunger," Says Nobel Laureate Sir Richard J. Roberts
- Arabidopsis HTT2 Improves Thermotolerance in Heading Chinese Cabbage
- OsIPMS1 Plays Key Role in Seed Vigor of Rice
Plant Breeding Innovations
- Application of CRISPR-Cas9 in China's Medicinal Yam
- Ms45 Homeologs Hold Key to Male Sterility in Wheat
- AgriLife Scientists Use Gene Knock in Approach for Broad Disease Resistance in Food Crops
Beyond Crop Biotech
- Chinese Scientists Complete Genome Sequencing of Tropical Sage Plant
- Scientists Develop Biological Switch to Control Genome Editing
- PagFBL1 Promotes Adventitious Root Formation in Poplar
From the BICs
- PABIC Organizes Science Writing Seminar
- Global Congress on Biotechnology
Subscribe to CBU: