Researchers Test TALEN Genome Editing on Peanut
May 23, 2018| |
Transcription activator like effector nucleases (TALENs), which allow the precise editing of DNA, have been developed and applied for genome engineering in various organisms. However, they are rarely used in higher plants, especially in allopolyploid plants. The team of Shijie Wen from Guangdong Academy of Agricultural Sciences, China performed targeted mutagenesis using TALENs in peanut (Arachis hypogaea).
Using TALENs, the team induced targeted mutations in the peanut fatty acid desaturase 2 (AhFAD2) gene. AhFAD2 mutations were identified in about 14% of the regenerated plants. The mutations in the mutant lines were also significantly correlated to oleic acid accumulation. Mutant lines exhibited a 0.5–2 fold increase in the oleic acid content compared with control plants.
The study suggests that TALEN-mediated targeted mutagenesis could be applied to peanut and could increase the oleic acid content in edible peanut oil.
For more information, read the article in Plant Molecular Biology.
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