TALENs-mediated Editing in Potato via AgroinfiltrationFebruary 14, 2018
A rapid test and an effective delivery system for introducing TALENs to plants are critical for successful target mutation. TALENs have usually been tested in protoplasts or introduced to plants with viral vectors. However, plant regeneration from protoplast culture can be unreliable. Viral vectors are not always available, and plants edited by these vectors require virus elimination.
Jin Ma from the Fredericton Research and Development Centre in Canada used a non-viral, Agrobacterium-mediated transient expression approach, to serve as both a rapid test and an effective delivery of TALENs into two vegetatively propagated potato (Solanum tuberosum) cultivars. Two TALENs were expressed to target two genes, one for a starch branching enzyme, and one for an acid invertase, into leaves of potato plants via agroinfiltration.
The infiltrated leaf DNA was analyzed and revealed that the two TALENs successfully agroinfiltrated and induced mutations at both targeted loci.
For more information on this study, read the article in Plant Biotechnology Reports.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Nigeria is Gearing towards Commercializing GM Seeds
- Genetic Trigger Adds Branches to Plants, Could Boost Crop Yields
- Australian OGTR Approves GM Cotton (COT102) and Canola (DHA Canola)
- USDA FAS-GAIN Reports Advancement of Agri-biotech in Bangladesh
- Found: Gene that Confers Resistance to Septoria
- Temperature Resilient Crops Now an "Achievable Dream"
- German Study Analyzes Risk Perceptions of Consumers Regarding GM Crops
- Rice Gene Makes Maize Productive; To Do the Same for Other Crops
- Scientists Conclude Safety of Pseudomonas as Source of Genes for GM Crops
- RTD1 Involved in Tocopherol Biosynthesis and Plant Development in Rice
Beyond Crop Biotech
- Pheromone from Asian Citrus Psyllid that Transmits Citrus Greening Now Identified
- Scientists Sequence Genomes of 60 Citrus Varieties to Draw Up Family Tree and Understand Disease Response
- TcCHS Gene Confers Strong Resistance Against Cotton Aphids
- Science and She Campaign
- Researchers Test CRISPR-Cas9 on Red Sage
- OsPKS2 Controls Rice Male Fertility by Regulating Pollen Wall Formation
- TALENs-mediated Editing in Potato via Agroinfiltration
- Scientists Study the Function of OsPT4 in Arsenic Uptake in Rice
Read the latest:
- Biotech Updates (November 29, 2023)
- Gene Editing Supplement (November 29, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: