Overexpression of BoC3H Gene Enhances Salt Stress Tolerance in BroccoliJuly 26, 2017
C3H-type zinc finger proteins have been known to play important roles in plant growth, development, and stress responses. The team of Ming Jiang of Taizhou University in China aimed to study a C3H-type zinc finger gene from broccoli (Brassica oleracea var. italica) designated as BoC3H.
The transcripts of BoC3H were found to be induced by NaCl, with the highest expression levels observed at 18h after treatment. Four broccoli lines overexpressing BoC3H gene were then developed. These lines exhibited higher germination rates, dry weight and chlorophyll content in response to salt stress compared to wild type plants.
Further analysis showed that overexpression of BoC3H significantly decreased hydrogen peroxide levels, relative electrical conductivity (REC) and malondialdehyde (MDA) contents, while dramatically increasing free proline content, catalase, peroxidase and superoxide dismutase enzyme activities. These resulted in less cell death in the leaves of transgenic plants.
These results suggest that BoC3H contributes to salt stress tolerance by regulating hydrogen peroxide levels, REC, free proline, MDA, and antioxidant enzyme levels in broccoli.
For more information, read the article in Plant Cell, Tissue and Organ Culture.
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Web Portal Established to Speed up Genetic Research in Plants
- India's B.R. Barwale Passes Away
- African Biosafety Regulators Embrace Biosafety Communication at ABBC 2017
- Biotechnology Praised at the Biggest Agri Expo in Uganda
- Adoption of GE Crops in the US
- New Gene in Corn Confers Resistance to Multiple Diseases
- Study Finds Plants Reprogram Their Genetic Material to Fight Pathogens
- China Gives Import Approval for Corn Rootworm Resistant Trait
- Russia Drafts Regulations for GE Feed
- Overexpression of BoC3H Gene Enhances Salt Stress Tolerance in Broccoli
- Study on the Effect of Light and Temperature on CBF14 Expression in Wheat and Barley
Beyond Crop Biotech
- NAS Announces Awards for Science Communication
- Researchers Develop Plant-produced Vaccine Candidates Against Bluetongue Virus
- CRISPR-Cas9 and CRISPR-Cpf1 Mediated Genome Editing of EPFL9 Gene in Rice
- Discovery on How CRISPR Proteins Identify Targets
Read the latest:
- Biotech Updates (March 22, 2023)
- Genome Editing Supplement (March 22, 2023)
- Gene Drive Supplement (February 22, 2023)
Subscribe to BU: