Genome Editing of Citrus Susceptibility Gene CsLOB1 Confers Resistance to Citrus CankerJanuary 11, 2017
Citrus production has many biotic challenges, such as bacterial canker and Huanglongbing (HLB), and using resistant varieties is the most effective way of controlling these diseases. However, traditional breeding of citrus is challenging due to polyploidy and long crossing cycles.
Targeted genome editing technology can potentially shorten the development of resistant citrus varieties. Using CRISPR/Cas9 technology, scientists from the University of Florida edited the canker susceptibility gene, CsLOB1, in Duncan grapefruit. Six independent lines, DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, were generated.
When inoculated with the canker pathogen Xanthomonas citri subsp. citri (Xcc), DLOB2 and DLOB3 showed symptoms similar to wild-type grapefruit. No canker symptoms were observed on DLOB9, DLOB10, DLOB11 and DLOB12 at 4 days after inoculation.
Pustules caused by Xcc were also observed on DLOB9, DLOB10, DLOB11 and DLOB12 in later stages, but were significantly less compared to pustules in wildtypes. Furthermore, the pustules on DLOB9 and DLOB10 did not develop into typical canker symptoms.
No side effects or off-target mutations were detected in the mutated plants.
For more information, read the full article in Plant Biotechnology Journal.
The Crop Biotech Update is a weekly newsletter of ISAAA, a not-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- Governments to Integrate Biodiversity Conservation Across Agri Sectors
- Study Presents Evidence on Movement of Potato Famine Pathogen
- Researchers Crack Genetic Basis of Cotton Leaf Shape
- Donald Danforth Plant Science Center, NRGene Assemble Second Maize Reference Genome
- Cuba to Plant GM Soybean and Corn in Spring 2017
- ICAR NBPGR Unearths Genetic Potential of Wheat Germplasm
- MicroRNA 157 Regulates Floral Organs Development and Ovule Production in Cotton
- Triple Gene Construct Confers Insect Resistance and Herbicide Tolerance in Tobacco
Plant Breeding Innovations
- Genome Editing of Citrus Susceptibility Gene CsLOB1 Confers Resistance to Citrus Canker
- CRISPR/Cas9-Induced Mutations in SELF PRUNING 5G Promotes Day-neutrality and Early Yield in Tomato
From the BICs
- Agricultural Biotechnology and its Potential Application in Vietnam
- Help Evaluate GMO Peer-reviewed Literature
- IPBO & IIBN Release Book on "Innovative Farming and Forestry Across the Emerging World: The Role of Genetically Modified Crops and Trees"
- ISAAA Blog: Trending News on Crop Biotech in 2016
Read the latest:
- Crop Biotech Update (May 25, 2022)
- Genome Editing Supplement (May 18, 2022)
- Gene Drive Supplement (May 25, 2022)
Subscribe to CBU: