Geminivirus-Mediated Genome Editing in Potato Using Sequence-Specific NucleasesSeptember 14, 2016
Genome editing using sequence-specific nucleases (SSNs), such as zinc finger nucleases (ZFNs), TALENs, and CRISPR/Cas is rapidly being improved for genetic engineering in crops. While non-homologous end-joining (NHEJ) has been the preferred DNA repair in plants, gene targeting via homologous recombination (HR) is more precise and allows incorporation of new sequence, and could be a powerful tool for directed DNA repair.
The team of Nathaniel M. Butler from Michigan State University used a geminivirus replicon (GVR) to deliver SSNs targeting the potato ACETOLACTATE SYNTHASE1 (ALS1) gene and repair templates designed to add herbicide-inhibiting point mutations within the ALS1 locus. The team used a geminivirus replicon due to its ability to produce a DNA replicon which can act as a repair template, as well as its relatively large carrying capacity.
Transformed events modified with GVRs held point mutations capable of supporting a reduced herbicide susceptibility phenotype, while plants modified with conventional T-DNAs were just similar to wild-types. Regeneration of transformed events improved detection of point mutations that supported a stronger reduced herbicide susceptibility phenotype.
These results show the effective use of geminiviruses in genome editing, and describes a new approach to gene targeting in vegetatively propagated species.
For more on this study, read the article in Frontiers in Plant Science.
The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization. The CBU is distributed for free to over 23,000 subscribers worldwide to inform them about the key developments in biosciences, especially in agricultural biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
See more articles:
News from Around the World
- FAO Projects Increase in Maize, Wheat, and Rice Grain Output
- Kanayo Nwanze Receives Inaugural Africa Food Prize
- Uganda's Minister of S&T Lauds Researchers on Biotech Innovations
- Kenya's National Biosafety Authority Gives GM Cotton Partial Nod
- Scientists Develop New Approach to Genetic Modification of Maize and Other Grains
- US EPA Approves New Agrisure® Trait Stack from Syngenta
- New Wheat Varieties with Dwarf Genes Show Promise
- South Asia Biotechnology Centre Invites Public Comments to Support GE Mustard
- Study Highlights Impact of Plant Breeding in the EU
- Fern Protein Expression in Cotton Prevents Whitefly Attack
- Researchers Search for Drought-Related Transcription Factors in Common Bean
Plant Breeding Innovations
- Geminivirus-Mediated Genome Editing in Potato Using Sequence-Specific Nucleases
- Researchers Engineer Potyvirus Resistance in Arabidopsis Using CRISPR/Cas9
Beyond Crop Biotech
- Trehalase Genes Regulate Expression of the Chitin Synthesis in Red Flour Beetle
- Biotech Crop Annual Updates and Biotech Trait Annual Updates
Subscribe to CBU: