Biotech Updates

Overexpression of DAAO Gene from a Bacterium Enhances Glyphosate Resistance in Arabidopsis

November 18, 2015

The d-amino acid oxidase (DAAO) can metabolize glyphosate and yield aminomethyl phosphonic acid and glyoxylate, which are less toxic to plants than glyphosate. However, studies on the use of DAAO to enhance glyphosate resistance in plants are lacking.

A research team led by Hongjuan Han of the Shanghai Academy of Agricultural Sciences in China reports the overexpression of the DAAO gene from the nitrogen-fixing bacterium, Bradyrhizobium japonicum, in Escherichia coli and Arabidopsis thaliana. To confirm the glyphosate resistance of the DAAO gene, the gene was transformed into E. coli. The positive E. coli transformants grew well both on solid and in liquid medium containing glyphosate.

The DAAO gene was then transformed into Arabidopsis thaliana. Nine days after application of 10 mM glyphosate, wild-type plants all died while transgenic plants grew normally.

For more information on the study, read the article on Plant Cell Reports.